Poster
# 86
Poster |
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6th Internet World Congress for Biomedical Sciences |
Kirsten Culver(1), Henry Szechtman(2)
(1)(2)McMaster University - Hamilton. Canada
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[Neuroscience] |
To induce locomotor sensitization, 30 male rats were injected twice weekly with quinpirole (0.5 mg/kg, s.c.) for a total of 8 injections, while 10 control rats were similarly injected with saline. Quinpirole-treated rats were assigned, at random, into 3 groups (n=10 per group): a chronic clorgyline group that received clorgyline (1 mg/kg/day) via osmotic mini-pump (QNP+CLG), a chronic clorgyline group that received a subcutaneous injection of clorgyline (1 mg/kg) 90 minutes prior to each quinpirole injection (QNP+CLGinj), and a quinpirole control group that received a subcutaneous injection of saline 90 minutes prior to each quinpirole injection (QNP+SAL). Saline control rats received a subcutaneous injection of saline 90 minutes prior to each of the 8 injections of saline (SAL Control).
Immediately following each injection of quinpirole, rats were placed in activity monitors and their locomotor activity was recorded for 90 minutes. During the first and last 15 minutes of the testing period, mouthing activity was recorded for 30 seconds every 3-3.5 minutes for a total of 4 minutes using a hand-held timer.
To determine whether chronic clorgyline blocked the induction of locomotor sensitization to quinpirole, or merely blocked its expression, clorgyline treatment was discontinued after the 8th quinpirole injection, and all groups received a test injection of quinpirole (0.5 mg/kg, s.c.) one week later.
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[Neuroscience] |
