Nina M. van Sorge⁽¹⁾, JP Yska⁽²⁾, Hans Blansjaar⁽³⁾, Sandra Driessen⁽⁴⁾, Patricia Schepers⁽⁵⁾, Simon R. Bovenga⁽⁶⁾, A.A. van Sorge^{(7)
(1)(2)(3)(4)(5)(6)}Rijnstate Hospital - ARNHEM. Netherlands
⁽⁷⁾Rijnstate Hospital Arnhem - ARNHEM. Netherlands

	[Pharmacology]	[Orthopedics & Traumatology]

MATERIALS AND METHODS

Dynamic elution profile

To study the elution profile of the gentamicin loaded surgical cement we used a USP XXVI defined flow-through cell apparatus. The assembly consists of a 600 ml reservoir (fig.1) containing 250 ml buffered solution; a pump for the dissolution medium; a flow-through cell (fig 2); a waterbath that maintains the dissolution medium at 37 ± 0,5 °C, a device for automatic sampling of the eluate.

Flow rate of the dissolution medium: 84 ml/hr.

Dissolution medium: buffered phosphate solution pH 7,5 ± 0,1 (KH₂PO₄ 1.82 g/l; Na₂HPO₄.2H₂O 9.5 g/l).

Specialties were obtained from Schering-Plough (Palacos®; Lotnr.:98D06-12/8928/97I09-46/2895) and Merck, Ortomed (Palamed®; Lotnr.: 8948 5054).

During the 190 min. test every 5 min. a sample of 250 uL was taken.

Non-dynamic time-elapsed elution profile

A final elution procedure (190 days) was studied by means of emersion of a sample of the gentamicin loaded cement in the same elution medium in an erlenmeyer (flask); appropriately diluted test samples were taken at four previously determined time points.

Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIALS AND METHODS] [RESULTS] [FIGURES] [DISCUSSION] [CONCLUSION] [BIBLIOGRAPHY] [PRESENTATION FILE] [Discussion Board]

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	[Pharmacology]	[Orthopedics & Traumatology]

Nina M. van Sorge, JP Yska, Hans Blansjaar, Sandra Driessen, Patricia Schepers, Simon R. Bovenga, A.A. van Sorge
Copyright © 1999-2000. All rights reserved.

Last update: 11/01/00