Poster
# 59
Poster |
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6th Internet World Congress for Biomedical Sciences |
Bettina Bert(1), Andre Rex(2), Heidrun Fink(3)
(1)Institute of Pharmacology and Toxicology. Humboldt-University Berlin, Charite - Berlin. Germany
(2)Institute of Pharmacology and Toxicology. VB Vterinary medicine, Free University - Berlin. Germany
(3)Institute of Pharmacology and Toxicology. School of Veterinary Medicine, Free University Berlin - Berlin. Germany
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[Neuroscience] |
[Pharmacology] |
Animals: Male Wistar rats (Harlan-Winkelmann and Federal Institute for Healthprotection of consumers and Veterinary medicine, FRG ) and Fischer 344 rats (Harlan-Winkelmann, FRG) 220 ± 30 g, were group housed under a 12 hr light-dark-schedule with food and water freely available. N = 8-10 per group.
Methods: A. Behavioural test
For the investigation of the anxiety-related behaviour the elevated plus maze test was used. The duration of the test was 5 min. The following parameters were measured: Time spent in the open arms, percentage of entries into the open arms, number of head dips and locomotor activity. 30 min before the start of the test the animals received an i.p. injection of saline or diazepam at a dosage of 0.5-4.0 mg/kg.
B. Plasma concentration
The plasma concentrations of diazepam, nordiazepam, temazepam and oxazepam were measured. The animals received an i.p. injection of 2.0 mg/kg diazepam and after 30 min intracardially blood samples were taken. The concentrations were measured by HPLC.
Statistics: All data are shown as means ± SEM. Statistical analysis was performed with one way ANOVA on Ranks followed by Dunn’s Test. Differences of p<0.05 were considered statistically significant.
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[Neuroscience] |
[Pharmacology] |
