María Dolores Mayas-Torres⁽¹⁾, José Manuel Martínez-Martos⁽²⁾, María Jesús Ramírez-Expósito⁽³⁾, María Jesús García-López⁽⁴⁾, Isabel Prieto-Gómez⁽⁵⁾, Garbiñe Arechaga-Maza⁽⁶⁾, Manuel Ramírez-Sánchez^{(7)
(1)(2)(4)(5)(6)(7)}Unit of Physiology. University of Jaén - Jaén. Spain
⁽³⁾Unit of Physiology. University of Jaen - Jaén. Spain

	[Endocrinology]	[Neuroscience]	[Pharmacology]	[Physiology]	[Toxicology]

RESULTS

The presence of ethanol in an artificial CSF containing calcium or not on the basal pGluAP activity in synaptosomes from the brain cortex of mice, showed the following results: Ethanol in an artificial CSF containing calcium inhibits pGluAP activity (fig 1). In this way, ethanol 25 mM induces a significant inhibition (p<0.01) of 45.68% vs. the control. Ethanol 50 mM reduces pGluAP activity in a 45.97% (p<0.01) and ethanol 100 mM causes an inhibition of 55.87% (p<0.01) vs. the control. In absence of calcium in the artificial CSF (fig 2), ethanol 25 mM produces a significant inhibition (p<0.01) on the pGluAP activity in a 49%. Ethanol 50 mM reduces the activity in a 55.19% (p<0.01) and ethanol 100 mM reduces pGluAP activity of 46.28% vs. the control (p<0.01).

The effects of ethanol and the influence of calcium in the artificial CSF on pGluAP activity after the stimulation of synaptosomes with K+ 25 mM showed the following results: In presence of calcium, the stimulation of synaptosomes with K+ 25 mM does not modify this activity (fig 2). In absence of calcium, the stimulation with K+ 25 mM reduces significantly the pGluAP activity in an 18.74% (p<0.01) vs. the control.

The simultaneous incubation, in an artificial CSF with calcium, of synaptosomes with K+ 25 mM and ethanol 25 mM (fig. 2) produces a significant decrease (p<0.01) of pGluAP in a 43.75% vs. the control. The presence of K+ 25 mM and ethanol 50 mM produce an inhibition of this activity in a 44.44% (p<0.01) vs. the control values. The stimulation of synaptosomes with K+ 25 mM in presence of ethanol 100 mM decreases (p<0.05) the pGluAP activity in a 7.54% vs. the control. In a calcium-free artificial CSF (fig.2), the stimulation of synaptosomes with K+ 25 mM and ethanol 25 mM produces a significant inhibition (p<0.01) in a 8.06% vs. the control. The stimulation with K+ 25 mM and ethanol 50 mM produces an inhibition (p<0.01) of 35.02% , and ethanol 100 mM of 40.34% (p<0.01) vs. the control.

Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION AND CONCLUSIONS] [BIBLIOGRAPHY] [Discussion Board]

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	[Endocrinology]	[Neuroscience]	[Pharmacology]	[Physiology]	[Toxicology]

María Dolores Mayas-Torres, José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Jesús García-López, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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Last update: 11/01/00