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6th Internet World Congress for Biomedical Sciences

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In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain.

María Dolores Mayas-Torres(1), José Manuel Martínez-Martos(2), María Jesús Ramírez-Expósito(3), María Jesús García-López(4), Isabel Prieto-Gómez(5), Garbiñe Arechaga-Maza(6), Manuel Ramírez-Sánchez(7)
(1)(2)(4)(5)(6)(7)Unit of Physiology. University of Jaén - Jaén. Spain
(3)Unit of Physiology. University of Jaen - Jaén. Spain

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION AND CONCLUSIONS] [BIBLIOGRAPHY] [Discussion Board]
DISCUSSION AND CONCLUSIONS Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. ABSTRACT
[Endocrinology]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.		 [Neuroscience]
Next: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.		 [Pharmacology]
Next: Reduced energy consumption by dexamethasone in the mouse heart.		 [Physiology]
Next: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.		 [Toxicology]
Next: DISTRIBUTION OF CADMIUM AND ITS EFFECTS ON THE ORGAN CONCENTRATIONS OF RETINOIDS AND BETA-CAROTENE

BIBLIOGRAPHY

  1. McKeon UG, O´Connor B. Mammalian Piroglutamyl-peptidase I. In Barrett AJ, Rowlings ND, Woesnner JF, eds. Handbook of Proteolytic Enzimes. London: Academic Press; 1998; 269: 796-7.
  2. Cummins PM, O´Connor B. Bovine brain pyroglutamyl aminopeptidase (Type-I): Purification and characterization of a neuropeptide-inactivating peptidase. Int J Biochem Cell Biol. 1996; 8: 883-93.
  3. Whittaker VP. The synaptosome. In Lajtha A, ed. Handbook of Neurochemistry, 2nd ed. New York: Ed. Plenum Press; 1984: vol 7.
  4. Schwabe C, McDonald JK. Demonstration of a pyroglutamyl residue at the N-terminus of the B-chain of porcine relaxin. Biochem Biophys Res Commun. 1977; 74: 1501-4.
  5. Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem. 1976; 72: 248-54.
  6. Mudge AW, Fellows RE. Bovine pituitary pyrrolidonecarboxylyl peptidase. Endocrinology. 1973; 93: 1428-34
  7. Browne P, O´Cuinn G. An evaluation of the role of a pyroglutamate aminopeptidase, a post-proline cleaving enzyme and a post-proline dipeptidyl aminopeptidase, each purified from the soluble fraction of guinea-pig brain, in the degradation of thyroliberin in vitro. Eur J Biochem. 1983; 137: 75-87.
  8. Lauffart B, McDermott JR, Biggins JA, Gibson AM, Mantle D. Purification and characterization of pyroglutamyl aminopeptidase from human cerebral cortex. Biochem Soc Trans. 1989; 17: 207-8.
  9. O´Connor B, O´Cuinn G. Localization of a narrow-specificity thyroliberin-hydrolysing pyroglutamate aminopeptidase in synaptosomal membranes of guinea-pig brain. Eur J Biochem. 1984; 144: 271-8.
  10. Elmore MA, Griffiths EC, O´Connor B, O´Cuinn G. Further characterization of the substrate specificity of TRH hydrolysing pyroglutamate aminopeptidase from guinea-pig brain. Neuropeptides 15, 31-36.
  11. Taylor WL, Dixon JE. Characterization of a pyroglutamate aminopeptidase from rat serum that degrades thyrotropin-releasing hormone. J Biol Chem. 1978; 253: 6934-40.
  12. Bauer K, Nowak P. Characterization of a thyroliberin-degrading serum enzyme catalyzing the hydrolysis of thyroliberin at the pyroglutamyl-histidine bond. Eur J Biochem. 1979; 99: 239-46.
  13. Bauer K, Nowak P, Kleinkauf H. Specificity of a serum peptidase hydrolysing thyroliberin at the pyroglutamyl-histidine bond. Eur J Biochem. 1981; 118: 173-6
  14. Bauer K. Purification and characterization of the thyrotropin-releasing-hormone-degrading ectoenzyme. Eur J Biochem. 1981; 224: 387-96.
  15. Luttinger D, Frye GD, Nemeroff CB, Prange AJ Jr. The effects of neurotensin, beta-endorphin, and bombesin on ethanol-induced behabiors in mice. Psychopharmacology-Berl. 1983; 79(4): 257-63.

Discussion Board
Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION AND CONCLUSIONS] [BIBLIOGRAPHY] [Discussion Board]
DISCUSSION AND CONCLUSIONS Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. ABSTRACT
[Endocrinology]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.		 [Neuroscience]
Next: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.		 [Pharmacology]
Next: Reduced energy consumption by dexamethasone in the mouse heart.		 [Physiology]
Next: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.		 [Toxicology]
Next: DISTRIBUTION OF CADMIUM AND ITS EFFECTS ON THE ORGAN CONCENTRATIONS OF RETINOIDS AND BETA-CAROTENE
María Dolores Mayas-Torres, José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Jesús García-López, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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