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6th Internet World Congress for Biomedical Sciences

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In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain.

María Dolores Mayas-Torres(1), José Manuel Martínez-Martos(2), María Jesús Ramírez-Expósito(3), María Jesús García-López(4), Isabel Prieto-Gómez(5), Garbiñe Arechaga-Maza(6), Manuel Ramírez-Sánchez(7)
(1)(2)(4)(5)(6)(7)Unit of Physiology. University of Jaén - Jaén. Spain
(3)Unit of Physiology. University of Jaen - Jaén. Spain

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION AND CONCLUSIONS] [BIBLIOGRAPHY] [Discussion Board]
RESULTS Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. DISCUSSION AND CONCLUSIONS
[Endocrinology]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.
[Neuroscience]
Next: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.
[Pharmacology]
Next: Reduced energy consumption by dexamethasone in the mouse heart.
[Physiology]
Next: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.
[Toxicology]
Next: DISTRIBUTION OF CADMIUM AND ITS EFFECTS ON THE ORGAN CONCENTRATIONS OF RETINOIDS AND BETA-CAROTENE

IMAGES

fig. 1
Figure 1. Effects of ethanol 25 mM, 50 mM and 100 mM on pGluAP activity in synaptosomes from the frontal cortex of mouse, incubated in presence or absence of calcium in the incubation buffer (artificial CSF). Results are expresed in nmol of pGlu-ß-naphthylamide hidrolyzed per min and per mg of protein (Mean±SEM; n=11; **p<0.01).

 

fig. 2
Figure 2. Effects of ethanol 25 mM, 50 mM and 100 mM on pGluAP activity in synaptosomes from the frontal cortex of mouse stimulated with K+ 25 mM, in presence or absence of calcium in the incubation buffer (artificial CSF). Results are expresed in nmol of pGlu-ß-naphthylamide hidrolyzed per min and per mg of protein (Mean±SEM; n=11; *p<0.05, **p<0.01).

 


Discussion Board
Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION AND CONCLUSIONS] [BIBLIOGRAPHY] [Discussion Board]

RESULTS Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. Previous: Effects of ethanol on aminopeptidase A in cortical synaptosomes. DISCUSSION AND CONCLUSIONS
[Endocrinology]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.
[Neuroscience]
Next: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.
[Pharmacology]
Next: Reduced energy consumption by dexamethasone in the mouse heart.
[Physiology]
Next: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.
[Toxicology]
Next: DISTRIBUTION OF CADMIUM AND ITS EFFECTS ON THE ORGAN CONCENTRATIONS OF RETINOIDS AND BETA-CAROTENE
María Dolores Mayas-Torres, José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Jesús García-López, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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Last update: 11/01/00