Satoshi Iwabuchi⁽¹⁾, Yasushi Sakai⁽²⁾, Hitoshi Kimura⁽³⁾, Shinya Yoshii⁽⁴⁾, Tetsuya Yokouchi⁽⁵⁾, Morikazu Ueda⁽⁶⁾, Hirotsugu Samejima^{(7)
(1)(3)(4)(5)(6)(7)}Department of Neurosurgery. Toho University - Tokyo. Japan
⁽²⁾Department of Physiology. Showa University College of Medical Sciences - Japan

Results

Contraction of smooth-muscle cells

Isolated smooth muscle cells contracted within 3 minutes after application of CSF (Figure 1). The application of CSF obtained on Day 3 following SAH induced greater contraction than that induced by CSF obtained on Day 7 (Figure 2). To test the involvement of protein in CSF with SAH, CSF pretreated with heating at 80�Ž was also applied in the same fashion. There was no significant difference between the contraction induced by application of CSF after heating and that induced by untreated CSF (Figure 3).

Intracellular Ca²⁺ mobilization

While CSF produced contraction of isolate smooth-muscle cells, the fluorescence signals of intracellular Ca²⁺ increased for 20-30 seconds after application of CSF, and then gradually decreased (Figure 4).

Effect of P2-purinoceptor antagonist on CSF-induced contraction

To test the involvement of nucleotides, suramin, an antagonist of P_2x and P_2y purinoceptors, was applied. Pretreatment of isolated smooth-muscle cells with suramin (100 ƒÊM) for 5 minutes caused a significant inhibition of the contraction (Figure 5).

Discussion Board

Any Comment to this presentation?

[ABSTRACT] [Introduction] [Materials & Methods] [Results] [Tables & Figures] [Discussion & Conclusion] [References] [Discussion Board]

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Satoshi Iwabuchi, Yasushi Sakai, Hitoshi Kimura, Shinya Yoshii, Tetsuya Yokouchi, Morikazu Ueda, Hirotsugu Samejima
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Last update: 19/01/00