Presentation
# 7
Presentation |
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6th Internet World Congress for Biomedical Sciences |
Satoshi Iwabuchi(1), Yasushi Sakai(2), Hitoshi Kimura(3), Shinya Yoshii(4), Tetsuya Yokouchi(5), Morikazu Ueda(6), Hirotsugu Samejima(7)
(1)(3)(4)(5)(6)(7)Department of Neurosurgery. Toho University - Tokyo. Japan
(2)Department of Physiology. Showa University College of Medical Sciences - Japan
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Contact address: |
Satoshi Iwabuchi Department of Neurosurgery Toho University 2-17-6, Ohashi, Meguro-ku Tokyo 1538515 Japan iwabuchi@med.toho-u.ac.jp |
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This study investigated the effect of cerebrospinal fluid (CSF) following subarachnoid hemorrhage (SAH) on isolated smooth-muscle cells. The application of CSF obtained on Day 3 following SAH induced a large contraction. An increase in the intracellular Ca2+ concentration of smooth-muscle cells was observed during contraction. There is a discrepancy between this observation and the fact that the peak of clinical vasospasm occurs about 7 days after hemorrhage. Following SAH, CSF may take a few days to affect smooth-muscle cells in the media of cerebral arteries.
There was no significant difference between the contraction induced by application of CSF after heating and that induced by untreated CSF. This result suggests that substances other than protein or peptides are related to vasospasm following SAH.
Preincubation of cells with suramin, an antagonist of P2x and P2y purinoceptors, attenuated the contraction after application of CSF, suggesting that nucleotides may be involved in the contraction of vascular smooth-muscle cells after SAH.
In conclusion, the contraction of cerebral smooth-muscle cells plays an important role in vasospasm following SAH, and this contraction may involve an increase of the intracellular Ca2+ concentration mediated by nucleotides.
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