Peter Massanyi⁽¹⁾, Laszlo Bardos⁽²⁾, Robert Toman⁽³⁾, Svatoslav Hluchy⁽⁴⁾, Jaroslav Kovacik⁽⁵⁾, Norbert Lukac^{(6)
(1)(3)(4)(6)}Slovak Agricultural University - Nitra. Slovakia
⁽²⁾University of Agricultural Sciences - Godollo. Hungary
⁽⁵⁾Slovak University of Agriculture - Nitra. Slovakia

	[Toxicology]	[Reproduction Sciences]

RESULTS

After a single intraperitoneal administration cadmium mainly accumulated in the liver. This accumulation is dose dependent (Table 1.), and proved to be significant in comparison with the control group (P<0.001). In the kidney cadmium levels significantly increased similarly to the liver. When evaluating muscle (m. quadriceps femoris), the cadmium concentration was in control 0.125 mg/kg, higher in group A 0.144 mg/kg and the highest in group B - 0.636 mg/kg. Low levels of cadmium were found in spleen (0.026 - 0.414 mg/kg). In ovary, cadmium concentration in control animals was under the detectable limit of 0.01 mg/kg, but a highly significant (P<0.001) increase after cadmium administration (1.835; 2.134 mg/kg, respectively) is reported. Very similar accumulation of cadmium has been observed in testis, and is dose dependent.

Determination of concentration of retinyl palmitate, retinol and ß-carotene is listed in Table 2. No significant differences were found in the liver, and the concentration of retinyl palmitate was 16.96 - 18.18 µg/g. In kidney, the level of retinyl palmitate was 15.05 - 16.81 µg/g. Retinol concentration significantly decreased only in the group with higher cadmium concentration - group B (P<0.001). Level of ß-carotene was significantly decreased in kidney and testis in both Cd-treated groups (P<0.001). In testis all parameters were affected. Significant decrease of retinyl palmitate and retinol was found in group B (P<0.001) in comparison with the control group. Analysis of ß-carotene showed significant decrease (P<0.001) in both groups with cadmium administration (0.85 and 0.41 µg/g, respectively) in comparison with control animals (1.48 µg/g).

Table 1. Distribution of cadmium (mg/kg wet tissue) in mice

 Organ         Group K      Group A        Group B
------  -------------   -------------   --------------
Liver	0.052 ± 0.008	4.196 ± 0.357*	8.360 ± 0.711*
Kidney	0.355 ± 0.030	2.382 ± 0.202*	2.709 ± 0.230*
Muscle	0.125 ± 0.011	0.144 ± 0.021	0.636 ± 0.145
Spleen	0.026 ± 0.002	0.026 ± 0.004	0.414 ± 0.035
Ovary	< 0.010	1.835 ± 0.156*	2.143 ± 0.182*
Testis	0.333 ± 0.028	0.450 ± 0.038	0.738 ± 0.063

* P<0.001 n = 32 Group K - control, untreated mice (n=10)
Group A - single i.p. dose 0.25 mg CdCl2.kg-1 (n=11)
Group B - single i.p. dose 0.5 mg CdCl2.kg-1 (n=11)

 

Table 2. Concentrations of retinyl palmitate, retinol and ß-carotene in mice liver, kidney and testis ( in µg/g)

					 
  Organ      Liver          Kidney         Testis
Group K -----------     ------------    ----------- 
RP	18.18 ± 1.85	15.05 ± 1.55	6.52 ± 0.78
ROL	26.39 ± 2.97	22.95 ± 1.98	8.21 ± 0.45
BC	1.95 ± 0.23	12.95 ± 1.33	1.48 ± 0.12

Group A  
RP	16.96 ± 1.55	16.81 ± 1.77	6.48 ± 0.78
ROL	15.61 ± 1.64	22.72 ± 2.23	8.27 ± 0.99
BC	3.19 ± 0.04	7.71 ± 0.77*	0.85 ± 0.09*

Group B
RP	17.55 ± 0.97	16.44 ± 1.98	3.03 ± 0.54*
ROL	13.24 ± 1.35	16.95 ± 1.55*	5.24 ± 0.26*
BC	3.24 ± 0.22	4.94 ± 0.58*	0.41 ± 0.05*

RP = retinyl palmitate, ROL = retinol, BC = ß-carotene (all values are expressed in µg/g)
Group K - control, untreated mice (n=10); Group A - single i.p. dose 0.25 mg CdCl2.kg-1 (n=11); Group B - single i.p. dose 0.5 mg CdCl2.kg-1 (n=11) *P<0.001; n = 32

Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]

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	[Toxicology]	[Reproduction Sciences]

Peter Massanyi, Laszlo Bardos, Robert Toman, Svatoslav Hluchy, Jaroslav Kovacik, Norbert Lukac
Copyright © 1999-2000. All rights reserved.

Last update: 10/01/00