Peter Massanyi⁽¹⁾, Laszlo Bardos⁽²⁾, Robert Toman⁽³⁾, Svatoslav Hluchy⁽⁴⁾, Jaroslav Kovacik⁽⁵⁾, Norbert Lukac^{(6)
(1)(3)(4)(6)}Slovak Agricultural University - Nitra. Slovakia
⁽²⁾University of Agricultural Sciences - Godollo. Hungary
⁽⁵⁾Slovak University of Agriculture - Nitra. Slovakia

	[Toxicology]	[Reproduction Sciences]

MATERIAL & METHODS

The experiments were conducted with 32 adult randombred ICR mice (Velaz Prague, Czech Republic) kept in plastic cages. Animals were divided into three groups (A, B, K). Mice in group A {11} were treated with a single i.p. cadmium dose of 0.25 mg CdCl2 (Sigma Chemical Company, St. Louis, MO, USA) per kg body weight, and were killed 48 hours later. Animals {11} in group B received a single i.p. dose of 0.5 mg CdCl2/kg b.w and were killed 48 hours after cadmium administration. The control group (K) consisted of 10 untreated mice. From all animals kidneys, liver, spleen, muscle (m. quadriceps femoris), ovaries and testes were collected. Concentrations of cadmium were determined by electrotermic atomic absorption spectrophotometry (Ekologické a veterinárne laboratóriá, Spišská Nová Ves, Slovak Republic, Unicam Solar 929). Aspiration method ⁽⁴⁾ of the ash solution with 1% HNO3, and then read at 228.8 µm wavelenght, following the Unicam AAS methods manual {1991} was used.

Concentrations of retinyl palmitate, retinol and ß-carotene were measured in all {32} animals. Retinoid content (retinyl palmitate, retinol) of kidney, liver and testes was extracted by n-hexane containing antioxidant (50 mg/l BHT). The extract was injected onto Si-100 S 10 CN column (BST Ltd., Budapest, Hungary). The elution parameters were as follows: mobile phase n-hexane: methanol {99.5 : 0.5}, flow rate 1.51 ml/min at 50 bar. The UV detection was carried out at 325 nm. Concentrations of b-carotene were measured in a similar arrangement but the detection was carried out at 450 nm. The peaks were identified by standard compounds : retinol (Sigma Chemical Company, St. Louis, MO, USA), retinyl palmitate (NBC, Cleveland, USA) and b-carotene (Merck, Darmstadt, FRG). The concentrations were determined from the peak-heights obtained by measuring the standard dilutions. From final data, basic statistical characteristics were calculated (mean, s.d.) and an analysis of variance by Sheffe´s test was completed for each variable.

Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]

---

	[Toxicology]	[Reproduction Sciences]

Peter Massanyi, Laszlo Bardos, Robert Toman, Svatoslav Hluchy, Jaroslav Kovacik, Norbert Lukac
Copyright © 1999-2000. All rights reserved.

Last update: 10/01/00