Sergej P. Osinsky, M.D.,⁽¹⁾, I Levitin⁽²⁾, L Bubnovskaya⁽³⁾, A Sigan⁽⁴⁾, I. Ganusevich⁽⁵⁾, V. Michailenko⁽⁶⁾, T. Kovelskaya^{(7)
(1)(2)(3)(4)(5)(6)(7)}Inst. exp. Pathol. Oncol. Radiobiol. - Kiev . Ukraine

	[Biophysics]	[Oncology]

MATERIALS AND METHODS

Compounds. Some cobalt(III) complexes with basic structure [ Co(acac₂en)(NH₃)₂] Cl that are containing no metal-carbon bond, and their analogs with different ligands were tested in vivo. Here they are exemplified by AC-11, AC-30 and AC-40 complexes ⁽⁸⁾. They were dissolved in aqua pro injectionibus immediately before use and injected intraperitoneally (mice) or intravenously (rat).

Tumors. Transplanted rat (Guerin carcinoma, Walker-256 carcinoma) and mice [Lewis lung carcinoma (3LL), melanoma B16, adenocarcinoma Ca755] tumors were used in this study. Tumors were transplanted into the right flank (rat) or intramuscularly into the leg (mice). Moreover, 3LL was transplanted into the pad of hind leg in experiments with amputation of the leg with primary tumors. The principles and methods of transplantation were conventional. Animals were kept in Makrolon cages bedded with dust-free wood granulate, and had free access to a standard diet and tap water. Rats treated with hyperthermia and radiation were anaesthetized with sodium pentobarbital (45 mg/kg i.p.). All experiments had been approved by the regional animal ethics committee.

Treatments. Local hyperthermia (LHT) (1 h, 43 or 41⁰C) was performed by microwave unit Luch-2 ("Medradioelectronics", Ukraine) operating at 2450 MHz with a 20 W power output. Each LHT treatment, either single or combined, was three-fold with a two-days break. The details of microwave heating and temperature measurement were described earlier ⁽¹⁰⁾. Irradiation (RT) was carried out using a 190 kV (current 10 mA, HVL= 0.5 mm Cu+1 mm Al) X-ray machine (RUM-17, Russia) at a dose of 1.23 Gy/min. Tumors were treated in a single exposure. Cobalt complexes were given 60 min before LHT or RT onset. Doses and schedules of complexes using alone in the experiments without LHT or RT are presented in a definite tables. Tumor´s response to treatment was estimated by standard methods.

Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIALS AND METHODS] [RESULTS] [DISCUSSION] [CONCLUSIONS] [REFERENCES] [Discussion Board]

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	[Biophysics]	[Oncology]

Sergej P. Osinsky, M.D.,, I Levitin, L Bubnovskaya, A Sigan, I. Ganusevich, V. Michailenko, T. Kovelskaya
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Last update: 15/01/00