Poster
# 23
Poster |
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6th Internet World Congress for Biomedical Sciences |
José Manuel Martínez-Martos(1), María Jesús Ramírez-Expósito(2), María Dolores Mayas-Torres(3), María Jesús García-López(4), Isabel Prieto-Gómez(5), Garbińe Arechaga-Maza(6), Manuel Ramírez-Sánchez(7)
(1)(3)(4)(5)(6)(7)Unit of Physiology. University of Jaén - Jaén. Spain
(2)Unit of Physiology. University of Jaen - Jaén. Spain
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[Cell Biology & Cytology] |
[Endocrinology] |
[Neuroscience] |
[Physiology] |
Values (mean±SEM) of specific aminopeptidase activities in cultured astrocytes added with several concentrations of oleic and linoleic fatty acids and cholesterol are presented in Figure 1. Oleic and linoleic fatty acids decrease AlaAP activity in a concentration-dependent manner, whereas cholesterol does not modify AlaAP activity. Thus, oleic acid 1 µM decreases significantly (P<0.01) AlaAP activity in 12% under control, whereas oleic acid 10 µM and 100 µM inhibits AlaAP activity in 19% under control (P<0.01). Linoleic acid decreases AlaAP activity in 8% under control (P<0.05); 10 µM linoleic acid decreases AlaAP activity in 16% under control (P<0.01) and 100 µM linoleic acid inhibits AlaAP activity in 25% under control (P<0.01).
With regard to the ArgAP activity, oleic acid does not modify this activity, whereas linoleic acid decreases ArgAP activity in 43% (P<0.01) only with the higher concentration of fatty acid tested. On the contrary, cholesterol increases ArgAP activity in a concentration-dependent manner. Thus, cholesterol 10 µM increases ArgAP activity in 23% over control (P<0.01), and cholesterol 100 µM in 67% over control (P<0.01), although cholesterol 1 µM does not modify ArgAP activity.
Oleic acid modifies CysAP activity when low quantities are added to the culture medium. In this way, oleic acid 1 µM decreases CysAP activity in 23% under control (P<0.01), whereas oleic acid 10 µM decreases CysAP activity in 16% under control (P<0.01). On the contrary, oleic acid 100 µM does not modify CysAP activity. By other hand, linoleic acid does not modify CysAP activity at any of the concentrations tested, but cholesterol increases significantly CysAP activity. in a concentration-dependent manner. Thus, cholesterol 1 µM increases CysAP activity in 6% over control (P<0.01), cholesterol 10 µM increases in 16% over control (P<0.01) and cholesterol 100 µM increases in 34% over control.
LeuAP activity is modified by oleic acid, decreasing in 30% under control with oleic acid 1 µM, in 38% under control with oleic acid 10 µM and in 32% under control with oleic acid 100 µM (P<0.01 in all cases). On the contrary, linoleic acid does not modify LeuAP activity. Moreover, cholesterol does not modify LeuAP activity in cultured astrocytes except when a high concentration is added (cholesterol 100 µM), which increases slightly LeuAP activity (5%; P<0.01).
With regard to the TyrAP activity, oleic acid does not modify this activity, but linoleic acid decreases significantly TyrAP activity in 30% under control (P<0.01), independently of the concentration of linoleic acid added to the culture medium. On the contrary, cholesterol increases TyrAP activity in a concentration-inversely dependent manner. Thus, cholesterol 1 µM increases TyrAP activity in 64% over control, cholesterol 10 µM increases TyrAP activity in 53% over control and cholesterol 100 µM increases TyrAP activity only in 43% over control (P<0.01 in all cases).
Neither oleic acid nor linoleic acid modifies pGluAP activity at any of the concentration tested. On the contrary, cholesterol increases pGluAP activity in a concentration-dependent manner. Thus, cholesterol 1 µM increases pGluAP activity in 16% over control, cholesterol 10 µM increases in 25% over control, and cholesterol 100 µM increases pGluAP activity in 57% over control (P<0.01 in all cases).
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[Cell Biology & Cytology] |
[Endocrinology] |
[Neuroscience] |
[Physiology] |
