José Manuel Martínez-Martos⁽¹⁾, María Jesús Ramírez-Expósito⁽²⁾, María Dolores Mayas-Torres⁽³⁾, María Jesús García-López⁽⁴⁾, Isabel Prieto-Gómez⁽⁵⁾, Garbiñe Arechaga-Maza⁽⁶⁾, Manuel Ramírez-Sánchez^{(7)
(1)(3)(4)(5)(6)(7)}Unit of Physiology. University of Jaén - Jaén. Spain
⁽²⁾Unit of Physiology. University of Jaen - Jaén. Spain

Contact address:

José Manuel Martínez-Martos Unit of Physiology University of Jaén Faculty of Experimental and Health Sciences Jaén E-23071 Spain jmmartos@ujaen.es

	[Cell Biology & Cytology]	[Endocrinology]	[Neuroscience]	[Physiology]

ABSTRACT

Background/Aims: A number of studies have addressed the interaction between fatty acids and lipids with central nervous system peptides. Aminopeptidases (AP) are involved in the metabolism of proteins and in the regulation of circulating hormones and biologically active peptides in tissues. The aim of the present work is to study the behaviour of several AP expressed in whole cell primary cultures of astrocytes, after incubation with oleic and linoleic fatty acids and cholesterol in the culture medium. Methods: Alanine-AP, arginine-AP, cystine-AP, leucine-AP, tyrosine-AP and pyroglutamate-AP activities were analysed in cultured astrocytes using the corresponding aminoacyl-ß-naphthylamides as substrates. Results: Oleic acid inhibits alanine-AP, cystine-AP and leucine-AP activities, whereas linoleic acid inhibits alanine-AP, arginine-AP and tyrosine-AP activities. Neither oleic acid nor linoleic acid modifies pyroglutamate-AP activity. On the contrary, cholesterol increases arginine-AP, cystine-AP, leucine-AP, tyrosine-AP and pyroglutamate-AP activities, although does not modify alanine-AP activity. Conclusion: until now, has been extensively reported that fatty acids and peptides may interact at the level of synthesis, release, receptors and post-receptors events. The changes reported here clearly demonstrate that oleic and linoleic fatty acids and cholesterol can modulate peptides activity through their degradation route due to aminopeptidases, being each of them differentially regulated.

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Keywords: Aminopeptidases - Oleic acid - Linoleic acid - Astrocytes - Primary cultures -

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[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [ACKNOWLEDGEMENTS] [BIBLIOGRAPHY] [Discussion Board]

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	[Cell Biology & Cytology]	[Endocrinology]	[Neuroscience]	[Physiology]

José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Dolores Mayas-Torres, María Jesús García-López, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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Last update: 16/12/99