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6th Internet World Congress for Biomedical Sciences

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Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.

José Manuel Martínez-Martos(1), María Jesús Ramírez-Expósito(2), María Dolores Mayas-Torres(3), María Jesús García-López(4), Isabel Prieto-Gómez(5), Garbiñe Arechaga-Maza(6), Manuel Ramírez-Sánchez(7)
(1)(3)(4)(5)(6)(7)Unit of Physiology. University of Jaén - Jaén. Spain
(2)Unit of Physiology. University of Jaen - Jaén. Spain

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [ACKNOWLEDGEMENTS] [BIBLIOGRAPHY] [Discussion Board]
ACKNOWLEDGEMENTS Previous: Genetic suppression in the Drosophila eye morphological pathway. Molecular Characterization of the Om(1J)Su locus. Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. Previous: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes. Previous: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes. ABSTRACT
[Cell Biology & Cytology]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.		 [Endocrinology]
Next: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity.		 [Neuroscience]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.		 [Physiology]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.

BIBLIOGRAPHY

  1. Losa GA, Graber R, Chigorno V, Sonnino S: Interaction of sialogangliosides with the methyltransferase-1 of microsomes from normal and neoplastic human mammary gland. Int J Cancer 1992; 52-738-742.
  2. Nuñez EA: Nonesterified fatty acids: role in the molecular events linking endocrinology and oncology via nutrition. Tumor Biol 1987; 8:273-280.
  3. Naor Z: Is arachidonic acid a second messenger in signal transduction? Mol Cell Endocr 1991; 80:C181-C186.
  4. Sumida C, Graber R, Nuñez EA: Role of fatty acids in signal transduction: modulators and messengers. Prostagl Leuktr Ess Fatty Acids 1993; 48:117-122.
  5. Graber R, Sumida C, Nuñez EA: Fatty acids and cell signal transduction. J Lipid Mediators Cell Signalling 1994; 9:91-116.
  6. Yehuda S, Rabinovitz S, Carasso RL, Mostofsky D: Fatty acids and brain peptides. Peptides 1998; 19:407-419.
  7. Sanderink GJ, Artur Y, Schlele F, Gueguen R, Slest G: Alanine aminopeptidase in serum: biological variations and reference limits. Clin Chem 1988; 34:1422-1426.
  8. Hertz L, Juurling BHJ, Fosmark H, Schousboe A: Astrocytes in primary cultures; in Pfeiffer J (ed): Neuroscience approached through cell culture. Boca Raton, FL, CRC Press, 1982, vol 1, pp 175.
  9. Hertz L, Juurling BHJ, Fosmark H, Schousboe A: Preparation of primary cultures of mouse (rat) astrocytes; in Shahar A, Filogamo G, Vernadakis A (eds): Cookbook for culturing neuronal and glial cells. University of Chiety (Italy). Internat Soc Dev Neurosci 81.
  10. Renau-Piqueras J, Zaragoza R, de Paz P, Baguena-Cervellera R, Megias L, Guerri C: Effects of prolonged ethanol exposure on the glial fibrillary acidic protein-containing intermediate filaments of astrocytes in primary culture: A quantitative immunofluorescence and immunogold electron microscopic study. J Histochem Cytochem 1989; 37:229-240.
  11. Greenberg LJ: Fluorimetric measurement of alkaline phosphatase and aminopeptidase activities in the order of 10-14 moles. Biochem Biophys Res Commu 1962; 9:430-35.
  12. Schwabe C, McDonald, JK: Demonstration of a pyroglutamyl residue at the N-terminus of the B-chain of porcine relaxin. Biochem Biophys Res Commun 1977; 74:1501-1504.
  13. Wagner GW, Tavianini MA, Herrmann KM, Dixon JE: Purification and characterization of an enkephalin aminopeptidase from rat brain. Biochemistry 1981; 13:3884-3890.
  14. Schnebli HP, Phillipps MA, Barclay RK: Isolation and characterization of an enkephalin-degrading aminopeptidase from rat brain. Biochim Biophys Acta 1979; 569:87-98.
  15. Bradford MM: A rapid and sensitive method for quantification of microgram quantities of protein-dye binding. Anal Biochem 1976; 72:248-254.
  16. Marteinsdottir I, Horrobin DF, Stenfors C, Theodorsson E, Mathé AA: Changes in dietary fatty acids alter phospholipid fatty acid composition in selected regions of rat brain. Prog Neuro-Psychopharmacol & Biol Psychiat 1998; 22:1007-1021.
  17. Bourre JM, Bonneil M, Dumont O, Piciotti M, Nalbone G, Lafont H: High dietary fish oil alters the brain polyunsaturated fatty acid composition. Biochim Biophys Acta 1988; 1043:149-152.
  18. Philbrick DJ, Mahadevappa VG, Ackman RG, Holub BJ: Ingestion of fish oil or a derived n-3 fatty acid concentrate containing eicosapentaenoic acid (EPA) affects fatty acid compositions of individual phospholipids of rat brain, sciatic nerve and retina. Nutrition 1987; 117:1663-1670.
  19. Murphy MG: Effects of exogenous linoleic acid on fatty acid composition, receptor-mediated cAMP formation and transport functions in rat astrocytes in primary culture. Neurochem Res 1995; 11:1365-1375.
  20. Thompson ABR, Keelan M, Clandinin MT: Feeding rats a diet enriched with saturated fatty acids presents the inhibitory effectos of acute and chronic ethanol exposure on the in vitro uptake of hexoses and lipids. Biochem Biophys Acta 1991; 1084:122-128.
  21. Lazar DF, Medzihradsky F: Altered microviscosity at brain membrane surface induces distinct and reversible inhibition of opioid receptor binding. J Neurochem 1992; 59:1233-1240.
  22. Ramírez MJ, Martínez JM, Prieto I, Alba F, Ramírez M: Dietary supplementation with olive oil influences aminopeptidase activities in mice. Nutr Res 1998; 18:99-107.
  23. Martínez JM, Ramírez MJ, Prieto I, Alba F, Ramírez M: Influence of dietary supplementation with olive oil on pyroglutamyl-ß-naphthylamide hydrolysing activity in serum and different tissues of mice. Fol Biol 1998; 44:213-216.
  24. Martínez JM, Ramírez MJ, Prieto I, Alba F, Ramírez M: Sex differences and in vitro effects of steroids on serum aminopeptidase activities. Peptides 1998; 19:1637-1640.
  25. Jung-Testas I, Renoir M, Bugnard H, Greene GL, Baulieu EE: Demonstration of steroid hormone receptors and steroid action in primary cultures of rat glial cells. J Steroid Biochem Mol Biol 1992; 41:621-631.
  26. Mellon SH, Deschepper CF: Neurosteroid biosynthesis: genes for adrenal steroidogenic enzymes are expressed in the brain. Brain Res 1993; 629:283-292.
  27. Mitsuhashi N, Kato J: Modulation of steroid hormone receptors in the brain –inhibitory effect of fatty acids on the binding between cytosol progestin receptors in the rat cortex and R5020. Psychoneuroendocrinology 1987; 12:159-161.
  28. Del Cerro S, García Estrada H, García Segura LM: Neuroactive steroids regulate astroglia morphology in hippocampal cultures from adult rats. Glia 1995; 14:65-71;
  29. Makita K, Falck JR, Capdevila JH: Cytochrome P450, the arachidonic acid cascade and hypertension: new vistas for an old enzyme system. Faseb J 1996; 10:1456-1463.
  30. Catalan RE, Martínez AM, Aragones MD, Robles A, Miguel BG: Substance P induces alterations on cerebral lipids involved in membrane fluidity. Biochem Biophys Res Commun 1987; 144:232-237.
  31. Catalan RE, Martínez AM, Miguel BG, Robles A, Aragones MD: Dual mechanism of phosphatidylinositol hydrolysis by substance P in brain. Eur J Biochem 1988; 172:547-552.
  32. Shmalwko IP, Mikhaleva II: Antimetastatic effect of the delta-sleep peptide dureng stress in mice with Lewis lung carcinoma. Eksp Onkol 1988; 10:57-60.
  33. Chen H: Protective effect of thyrotropin-releasing hormone agaisnt lipid peroxidation injury during traumatic shock in rats. Chung Hua I Hsueh Tsa Chih 1991; 71:438-440.
  34. Judd AM, MacLeod RM: Thyrotrophin-releasing hormone and lysine-bradykinin stimulate arachidonate liberation from rat anterior pituitary cells through different mechanisms. Endocrinology 1992; 131:1251-1260.
  35. Naor Z: Signal transduction mechanisms of Ca2+ mobilizing hormones: the case of gonadotropin-releasing hormone. Endocr Rev 1990; 11:326-353.
  36. Noda Y, Rodriguez-Sierra J, Liu J, Landers D, Mori A, Paul S: Partitioning of vasoactive intestinal polypeptide into lipid bilayers. Biochim Biophys Acta 1994; 1191:324-330.
  37. Roundbaraki MM, Vacher P, Drouhault R: Arachidonic acid increases cytosolic calcium and stimulates hormone releas in rat lactotrophs. Am J Physiol 1995; E1215-1223.
  38. Señaris RM, Lewis MD, L
  39. Hargreaves K, Clandinin MT: Dietary lipids in relation to postnatal development of the brain. Ups J Med Sci 1990; 48:79-95.
  40. Murphy MG, Moak CM, Rao BG: Effects of membrane polyunsaturated fatty acids on opiate peptide inhibition of basal and prostaglandin E1-stimulated cyclic AMP formation in intact NIE-115 neurometabolism cells. Biochem Phamacol 1987; 36:4079-4084.
  41. Schwyzer R: Estimated membrane structure and receptor subtype selection of an opioid alkaloid-peptide hybrid. Int J Pept Protein Res 1988; 32:476-483.
  42. Myers M, Freire E: Colorimetric and fluorescence characterization of interactions between enkephalins and liposomal and synaptic plasma membranes containing gangliosides. Biochemistry 1995; 24:4076-4082.
  43. Barb CR, Kraeling RR, Rampacek, GB: Glucose and free fatty acid modulation of growth hormone and luteinizing hormone secretion by cultures porcine pituitary cells. J Anim Sci 1995; 73:1416-1423.
  44. Canonico PL: Growth hormone releasing factor (GRF) increases free arachidonate levels in the pituitary: a role for lipoxygenase products. Life Sci 1986; 38:267-272.
  45. Kennedy JA, Nicolson R, Wellby ML: The effect of oleic acid on the secretion of thyrotrophin and growth hormone by cultured rat anterior pituitary cells. J Endocrinol 1994; 143:557-564.
  46. Lyson K, McCann SM: Involvement of arachidonic acid cascade in interleukin-6-stimulated corticotropin-releasing factor release in vitro. Neuroendocrinology 1992; 55:703-708.
  47. Huang YS, Koba K, Horrobin DF, Sugano M: Interrelationship between dietary protein, cholesterol and n-6 polyunsaturated fatty acid metabolism. Prog Lipid Res 1993; 32:123-137.
  48. Horrocks LA, Harder HW: Fatty acids and cholesterol; in Lajtha A (ed): Handbook of neurochemistry. New York, Plenum 1983, pp1-16.
  49. Salem N Jr, Niebyski CD: The nervous system has an absolute molecular species requirement for proper function. Mol Membr Biol 1995; 12:131-134.
  50. Yehuda S, Carrasco RL: Modulation of learning pain thresholds, and thermoregulation in the rat by preparations of free-purified -linolenic and linoleic acids: determination of the optimal w3-to w6 ratio. Proc Natl Acad Sci USA 1993; 90:10345-10349.
  51. Hersh LB: Characterization of membrane bound aminopeptidases from rat brain: identification of the enkephalin-degrading aminopeptidase. J Neurochem 1985; 44:1427-1435.
  52. Ahmad S, Ward PE: Role of aminopeptidase activity in the regulation of the pressor activity of circulating angiotensins. J Pharmacol Exp Ther 1990; 252:643-650.
  53. McDonald JK, Barret AJ: Mammalian Proteases: A Glossary and Bibliography. Londres, Academic Press, 1986.
  54. Johnson GD, Hersh LB: Studies on the subsite specificity of the rat brain puromycin-sensitive aminopeptidase. Arch Biochem Biophys 1990; 276:305-309.
  55. McDermott JR, Mantle D, Lawfort B, Gibson AM, Biggins A: Purification and characterization of two soluble Cl- activated arginyl aminopeptidase from human brain and their endopeptidase action on neuropeptides. J Neurochem 1988; 50:176-82.
  56. Itoh C, Nagamatsu A: An aminopeptidase activity from porcine kidney that hydrolyzes oxytocin and vasopressin: purification and partial characterization. Biochim Biophys Acta 1995; 1243:203-208.
  57. Bauer K: Purification and characterization of the thyrotropin-releasing-hormone-degrading ectoenzyme. Eur J Biochem 1994; 224:387-396.
  58. Cummins PM, O´Connor B: Bovine brain pyroglutamyl aminopeptidase (type-I): purification and characterization of a neuropeptide-inactivating peptidase. Int J Biochem Cell Biol 1996; 8:883-893.
  59. Berg MJ, Marks N: Formation of des-tyr-dynorphins 5-17 by a purified cytosolic aminopeptidase of rat brain. J Neurosci Res 1989; 11:313-321.
  60. Greenfield SA, ShawLJ: Release of acetylcholinesterase and aminopeptidase in vivo following infusion of amphetamine into the sustantia nigra. Neuroscience 1982; 7:2823-2893.
  61. Lavado E, Sánchez-Abarca LI, Tabernero A, Bolaños JP, Medina JM: Oleic acid inhibits GAP junctions permeability and increases glucose uptake in cultured rat astrocytes. J Neurochem 1997; 69:721-728.

Discussion Board
Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [ACKNOWLEDGEMENTS] [BIBLIOGRAPHY] [Discussion Board]
ACKNOWLEDGEMENTS Previous: Genetic suppression in the Drosophila eye morphological pathway. Molecular Characterization of the Om(1J)Su locus. Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. Previous: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes. Previous: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes. ABSTRACT
[Cell Biology & Cytology]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.		 [Endocrinology]
Next: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity.		 [Neuroscience]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.		 [Physiology]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.
José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Dolores Mayas-Torres, María Jesús García-López, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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Last update: 16/12/99