Alberto Pizarro Gallardo^(1)
(1)Facultad de Medicina U.A.N. - Tepic. Mexico

	[Gastroenterology & Hepatology]	[Pathology]

MATERIALS & METHODS

We identified one case in the hospital : Centro Médico La Raza IMSS (México DF) Material of case was received unfixed. Tissue for examination by light microscopy was fixed in 10 % neutral buffered formalin embedded in paraffin, and stained with hematoxilin and eosin. Material for electron microscopy was obteined fresh and were fixed in phosphate buffered 5 % glutaraldehyde, postfixed in 1% osmium tetroxide and dehydrated in graded alcohols, embebbed in Epon-araldite 812. One micron thick sections stained with toluidine blue were used to select appropiated areas for study ultrathin sections were stained with uranyl acetate and lead citrate and examed in a Philips EM-300 Trasmission electron microscope.

The variables of study were : type of cell (round o fusiform), type of muscle (smooth or striated), type of nuclei(round, fusiform or irregular), number of nucleoli, giant cell( yes or not) rugous endoplasmic reticulum(yes o not), collagen(yes or not)microtubules (yes or not)and Number of mitoses x 10 high power field. All dates were captured in basedates Access 97 and analysed in Excell 97 and Epiinfo 6.04.

Discussion Board

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[ABSTRACT] [INTRODUCTION] [MATERIALS & METHODS] [RESULTS] [IMAGES] [IMAGES-2] [DISCUSSION] [CONCLUSION] [REFERENCES] [Discussion Board]

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	[Gastroenterology & Hepatology]	[Pathology]

Alberto Pizarro Gallardo
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Last update: 16/01/00