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6th Internet World Congress for Biomedical Sciences

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Influence of hormonal status on male mice aminopeptidase A activity.

María Jesús García-López(1), María Jesús Ramírez-Expósito(2), José Manuel Martínez-Martos(3), María Dolores Mayas-Torres(4), Isabel Prieto-Gómez(5), Garbiñe Arechaga-Maza(6), Manuel Ramírez-Sánchez(7)
(1)(3)(4)(5)(6)(7)Unit of Physiology. University of Jaén - Jaén. Spain
(2)Unit of Physiology. University of Jaen - Jaén. Spain

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION] [REFERENCES] [Discussion Board]
MATERIAL & METHODS Previous: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity. Previous: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity. Previous: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity. Previous: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity. Previous: REPRODUCTION TOXICOLOGY OF CADMIUM : A SCANNING ELECTRON MICROSCOPY STUDY IMAGES
[Biochemistry]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
[Endocrinology]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
[Neuroscience]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
[Physiology]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
[Reproduction Sciences]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.

RESULTS

Figure 1 shows Soluble and membrane bound AspAP activity. In serum, an increase of activity was observed after the injection of doses 1 (p<0.05; p<0.01) and 2 (p<0.01) of testosterone in relation with control group and ORX. Orchiectomy only reduce Sol AspAP activity in frontal cortex (p<0.01). Rest of tissues did not modified their activity. The levels of activity in frontal cortex after the injection of testosterone was maintained lower than in controls. The injection of dose 1 of testosterone increase significantly (p<0.01) the AspAP activity in hypophysis and hypothalamus. No changes were observed in membrane bound AspAP activity after orchiectomy.

Figure 2 shows soluble and membrane bound GluAP activity. In serum orchiectomy did not modified this activity, but the injection of doses 1 and 2 of testosterone increases significantly (p<0.01) GluAP activity. Soluble GluAP activity was decreased after orchiectomy in frontal cortex (p<0.05) and hypothalamus (p<0.05) and increased in hypophysis (p<0.01). The injection of testosterone reduce significantly (p<0.01) the levels of activity in hypothalamus. Membrane-bound GluAP activity was increased (p<0.01) after orchiectomy in Hypophysis but no changes were observed in rest of tissues.


Discussion Board
Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION] [REFERENCES] [Discussion Board]

MATERIAL & METHODS Previous: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity. Previous: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity. Previous: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity. Previous: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity. Previous: REPRODUCTION TOXICOLOGY OF CADMIUM : A SCANNING ELECTRON MICROSCOPY STUDY IMAGES
[Biochemistry]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
[Endocrinology]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
[Neuroscience]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
[Physiology]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
[Reproduction Sciences]
Next: Testosterone modifies arylamidase activities in serum and different tissues of mice: an in vivo study.
María Jesús García-López, María Jesús Ramírez-Expósito, José Manuel Martínez-Martos, María Dolores Mayas-Torres, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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Last update: 15/01/00