Poster
# 34
Poster |
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6th Internet World Congress for Biomedical Sciences |
Maria Rosa Sánchez(1), Manuel Samaniego(2)
(1)Departamento de Bioquímica.. Hospital Universitario "San Cecilio". Granada - Granada. Spain
(2)Unidad Neonatal. Hospital Universitario "Virgen de las Nieves". - Granada. Spain
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[Biochemistry] |
Study population: 62 normal subjects and 80 patients affected by different pulmonary diseases were grouped according to age as following:
1) 26 preterm neonates suffering Respiratory Distress Syndrome (RDS) requiring mechanical ventilation and 20 healthy newborns of similar gestational ages and birth weights.
2) 44 children, rangin from 2 to 12 years old: 12 healthy and 32 with respiratory problems (18 obstructive bronchitis from alergical causes, and 15 inflamatory process by infection agents).
3) 52 adults (30-65 years old): 30 control subjets and 22 patients with bronchopulmonary pathology and infiltrates of doubtful cause in chest radiograph, all of them examined by bronchoscopy for the purpose of diagnosis.
enzyme assays: The bronchial aspirate was collected by standard methods after bronchial lavage with saline solution at 0,9%, and centrifuged to eliminate mucus. The supernatant was used for the analysis. The total activity of ALP was assayed at 37ºC by a multiparametric analyzer Hitachi 917. The isoenzymes were separated by electrophoresis on agarose gel with Paragon Beckman System Isopal, using confirmatory methods (thermodenaturation, incubation with neuraminidase, ficin and urea, treatment with antiplacental antiserum, precipitation with lecitin of wheat germen), quantified by densitometry and their activity expressed as a percentage of the total ALP. Total proteins were quantified in the BALF and ALP enzymatic activity expressed in U/mg of protein × 10-³
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[Biochemistry] |
