Poster | 6th Internet World Congress for Biomedical Sciences |
Maria Rosa Sánchez(1), Manuel Samaniego(2)
(1)Departamento de Bioquímica.. Hospital Universitario "San Cecilio". Granada - Granada. Spain
(2)Unidad Neonatal. Hospital Universitario "Virgen de las Nieves". - Granada. Spain
Contact address: |
Maria Rosa Sánchez Departamento de Bioquímica. Hospital Universitario "San Cecilio". Granada Avda. del Dr Olóriz Granada Granada 18012 Spain rsanchez@hsc.sas.cica.es |
[Biochemistry] |
In this study, we are looking at the principal isoenzymes of alkaline phosphatase (ALP), as injury markers of cellular membranes from bronchial epithelium, in 80 patients diagnosed with bronchopulmonary pathology from different ethiology, using serum and bronchoalveolar lavage fluid (BALF) samples.
Patients were grouped according to age as following: 26 preterm neonates suffering respiratory distress syndrome (RDS) requiring mechanical ventilation; 32 children ranging from 2 to 12 years old, and 22 adults (30-65 years old) examined by bronchoscopy for the purpose of diagnosis. Results obtained from all pathological groups were compared with a control group showing similar characteristics.
Isoenzymes were separated by electrophoresis on agarose gel and were quantified by desitometry. Total protein was measured in BALF; ALP activity was expressed in UI/mg × 10-³ of protein.
We found that macromolecular ALP fraction was significantly increased in the serum of neonates with distress (p<0.01), in the patients 2-12 years and adults affected by pulmonary pathology (p<0.001). The electrophoresis of ALP isoenzymes showed a unique isoenzymatic band corresponding with its macromolecular fraction in the BAL fluid. We conclude that increased macromolecular fraction of alkaline phosphatase found in the serum of patients diagnosed with respiratory problems could have its origin in damaged pulmonary tissue.
[Biochemistry] |