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6th Internet World Congress for Biomedical Sciences

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Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.

José Manuel Martínez-Martos(1), María Jesús Ramírez-Expósito(2), María Dolores Mayas-Torres(3), María Jesús García-López(4), Isabel Prieto-Gómez(5), Garbiñe Arechaga-Maza(6), Manuel Ramírez-Sánchez(7)
(1)(3)(4)(5)(6)(7)Unit of Physiology. University of Jaén - Jaén. Spain
(2)Unit of Physiology. University of Jaen - Jaén. Spain

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [ACKNOWLEDGEMENTS] [BIBLIOGRAPHY] [Discussion Board]
RESULTS Previous: Genetic suppression in the Drosophila eye morphological pathway. Molecular Characterization of the Om(1J)Su locus. Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. Previous: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes. Previous: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes. DISCUSSION
[Cell Biology & Cytology]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.		 [Endocrinology]
Next: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity.		 [Neuroscience]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.		 [Physiology]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.

FIGURES

fig. 1
Figure 1. Specific AlaAP, ArgAP, CysAP, LeuAP, TyrAP and pGluAP activities in rat astrocytes in primary culture, after exogenous addition of oleic and linoleic fatty acids and cholesterol (1, 10 and 100 µM) complexed with methyl-ß-cyclodextrin, and controls (only with methyl-ß-cyclodextrin). Values represent mean±SEM levels of specific aminopeptidase activities, expressed as nmol of the corresponding aminoacyl-ß-naphthylamide hydrolyzed per min per mg of protein (n=8; *P<0.05; **P<0.01).

 

Discussion Board
Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [ACKNOWLEDGEMENTS] [BIBLIOGRAPHY] [Discussion Board]
RESULTS Previous: Genetic suppression in the Drosophila eye morphological pathway. Molecular Characterization of the Om(1J)Su locus. Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. Previous: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes. Previous: Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes. DISCUSSION
[Cell Biology & Cytology]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.		 [Endocrinology]
Next: In vivo effects of testosterone on mouse pyroglutamyl aminopeptidase activity.		 [Neuroscience]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.		 [Physiology]
Next: Quantitative changes in neuronal population during aging and contralateral lesions.
José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Dolores Mayas-Torres, María Jesús García-López, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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Last update: 16/12/99