José Manuel Martínez-Martos⁽¹⁾, María Jesús Ramírez-Expósito⁽²⁾, María Dolores Mayas-Torres⁽³⁾, Isabel Prieto-Gómez⁽⁴⁾, Manuel Ramírez-Sánchez^{(5)
(1)(3)(4)(5)}Unit of Physiology. University of Jaén - Jaén. Spain
⁽²⁾Unit of Physiology. University of Jaen - Jaén. Spain

	[Neuroscience]	[Physiology]

INTRODUCTION

In 1983, Mosmann used the tetrazolium salt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (MTT) to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay is based on cleavage of the yellow tetrazolium salt MTT, which forms water-insoluble dark blue formazan crystals. This cleavage takes place in cells by the mitochondrial enzyme succinate-dehydrogenase (Slater, Sawyer & Strauli, 1963). This assay detects living, but not dead cells, and the signal generated is dependent of the degree of activation of the cells. Since this report, MTT reduction is one of the most frequently used methods of measuring cell proliferation and cytotoxicity. Due to the extended use of synaptosomes to study physiological, biochemical and pharmacological aspects of synaptic function, our aim was to study the activity of mitochondrial respiratory chain by MTT assay, under resting and K+- and ATP- stimulated conditions in rat and mouse brain cortex synaptosomes, as an index of the functionality and energetic state of this subcellular fraction.

Discussion Board

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[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]

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	[Neuroscience]	[Physiology]

José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Dolores Mayas-Torres, Isabel Prieto-Gómez, Manuel Ramírez-Sánchez
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Last update: 16/12/99