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6th Internet World Congress for Biomedical Sciences

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Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.

José Manuel Martínez-Martos(1), María Jesús Ramírez-Expósito(2), María Dolores Mayas-Torres(3), Isabel Prieto-Gómez(4), Manuel Ramírez-Sánchez(5)
(1)(3)(4)(5)Unit of Physiology. University of Jaén - Jaén. Spain
(2)Unit of Physiology. University of Jaen - Jaén. Spain

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]
DISCUSSION Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. ABSTRACT
[Neuroscience]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.		 [Physiology]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.

BIBLIOGRAPHY

  1. Azbill, R.D., Mu, X., Bruce-Keller, A.J., Mattson, M.P. and Springer, J.E. (1997). Impaired mitochondrial function, oxidative stress and altered antioxidant enzyme activities following traumatic spinal cord injury. Brain Research 765, 283-290.
  2. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.
  3. Callahan, B., Yuan, J., Stover, G., Hatzidimitriou, G. and Ricaurte, G. (1998). Effects of 2-deoxy-D-glucose on methamphetamine- induced dopamine and serotonin neurotoxicity. Journal of Neurochemistry 70, 190-197.
  4. Curti, D., Izzo, E., Brambilla, L.,Facchetti, G., Sangiovanni, G. and Brambilla, G. (1995). Effect of ubiquinone like molecule on oxidative energy metabolism in rat cortical synaptosomes at different ages. Neurochemical Research 20, 1001-1006.
  5. Erecinska, M., Nelson, D., Deas, J. and Silver I.A. (1996). Limitation of glycolysis by hexokinase in rat brain synaptosomes during intense ion pumping. Brain Research 726, 153-159.
  6. Erecinska, M., Nelson, D. and Silver, J.A. (1996). Metabolic and energetic properties of isolated nerve ending particles (synaptosomes). Biochimica et Biophysica Acta 1277, 13-34.
  7. Erecinska, M., Zaleska, M.M., Chiu, L. and Nelson, D. (1991). Transport of asparagine by rat brain synaptosomes: an approach to evaluate glutamine accumulation. Journal of Neurochemistry 57, 491-498.
  8. Gabbita, S.P., Butterfield, D.A., Hensley, K., Shaw, W. and Carney, J.M. (1997). Aging and caloric restrictition affect mithocondrial respiration and lipid membrane status: an electron paramagnetic resonance investigation. Free Radical Biology and Medicine 23, 191-201.
  9. Hertz, L. and Peng, L. (1992). Effects of monoamine transmitters on neurons and astrocytes: correlation between energy metabolism and intracellular messengers. Progress in Brain Research 94, 283-301.
  10. Huang, H.M., Toral-Barza, L., Gibson, G. (1991). Cytosolic free calcium and ATP in synaptosomes after ischemia. Life Sciences 48, 1439-1445.
  11. Keller, J.N., Pang, Z., Geddes, J.W., Begley, J.G., Germeyer, A., Waeg, G. and Mattson, M.P. (1997). Impairment of glucose and glutamate transport and induction of mitochondrial oxidative stress and dysfunction in synaptosomes by amyloid beta-peptide: role of the lipid peroxidation product 4-hydroxynonenal. Journal of Neurochemistry 69, 273-284.
  12. Kyriazy, H.T. and Basford, R.E. (1986). Intractable unphysiologically low adenylate energy charge values in synaptosome fraction`s heterogeneity. Journal of Neurochemistry 47, 512-528.
  13. Langley, K. and Grant, N.J. (1997). Are exocytosis mechanisms neurotransmitter specific? Neurochemistry International 31, 739-757.
  14. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65, 55-63.
  15. Raatikainen, O., Kauppinen, R.A., Komulainen, H., Taipale, H., Pirttila, T. and Tuomisto, J. (1991). Polyene antibiotics increase the ionic permeability of synaptosomal plasma membranes. Biochemical Pharmacology 41, 1345-1350.
  16. Santos, M.S., Moreno, A.J. and Carvalho, A.P. (1996). Relationships between ATP depletion, membrane potential, and the release of neurotansmitters in rat nerve terminals. An in vitro sudy under conditions that mimic anoxia, hypoglucemia, and ischemia. Stroke 27, 941-950.
  17. Sheng, Z.H., Westenbroek, R.E. and Catterall, WA. (1998). Physical link and functional coupling of presynaptic calcium channels and the synaptic vesicles docking/fusion machynery. Journal of Bioenergetics and Biomembranes 30, 335-345.
  18. Slater, T.F., Sawyer, B. and Strauli, U. (1963). Studies on succionate-tetrazolium reductase systems. III. Points of coupling of four different tetrazolium salts. Biochimica et Biophysica Acta 77, 383-393.
  19. Struzynska, L., Dabrowska-Bouta, B. and Rafalowska, U. (1997). Acute lead toxicity and energy metabolism in rat brain synaptosomes. Acta Neurobiologiae Experimentalis Warsz 57, 275-281.
  20. Szutowicz, A., Tomaszewicz, M. and Bielarczyk, H. (1997). Key role of acetyl-CoA in cytoplasm of nerve terminals in disturbances of acetylcholine metabolism in brain. Folia Neuropathologica 35, 241-243.
  21. Whittaker, V.P., Michaelson, I.A. and Kirkland, R.J. (1964). The separation of synaptic vesicles from nerve-ending particles (`synaptosomes´). Biochemical Journal 90, 293-303.
  22. Zimmermann, H. (1994). Signalling via ATP in the nervous system. Trends in neurosciences 17, 420-425.

Discussion Board
Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]
DISCUSSION Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. ABSTRACT
[Neuroscience]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.		 [Physiology]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.
José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Dolores Mayas-Torres, Isabel Prieto-Gómez, Manuel Ramírez-Sánchez
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Last update: 16/12/99