Poster
# 19
Poster |
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6th Internet World Congress for Biomedical Sciences |
María Dolores Mayas-Torres(1), José Manuel Martínez-Martos(2), María Jesús Ramírez-Expósito(3), María Jesús García-López(4), Isabel Prieto-Gómez(5), Garbiñe Arechaga-Maza(6), Manuel Ramírez-Sánchez(7)
(1)(2)(4)(5)(6)(7)Unit of Physiology. University of Jaén - Jaén. Spain
(3)Unit of Physiology. University of Jaen - Jaén. Spain
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[Neuroscience] |
[Physiology] |
Under the experimental conditions used in the present work, the administration of ethanol to mouse frontal cortex synaptosomes, induces few signs of neurodegenerative processes. Using luminol as enhancer of the chemiluminescence signal, increases on free radical generation are not detected, but using lucigenin, a slight increase in chemiluminiscence appears in an inversely-proportional way to ethanol concentration. These free radicals seem to be the consequence of the increase of the mitochondrial activity, as demonstrated by MTT assay, but have not ability to induce degeneration, as demonstrated by their inability to produce peroxidation on the membrane lipids (even the smallest doses of ethanol seems to have a protective function at basal levels) or protein oxidation.
On the contrary, ethanol modifies significantly the level of several aminopeptidases. In basal conditions, ethanol inhibits these enzymatic activities in a dose-dependent manner or with a two-phase effect depending on ethanol concentration. In depolarizant conditions, ethanol produces an inhibition of these enzymatic activities in a dose-dependent manner, decreasing highly the levels of the enzymatic activity that were obtained with depolarization.
It is, therefore, evident, that ethanol modifies in a different way several aminopeptidase activities of synaptosomes from mouse cortex depending on the basal or depolarized conditions. Due to the fact that these enzymatic activities has been described as regulators of several neurotransmitters and/or neuromodulatory systems that are mediated by different types of neuropeptides (10), some of the ethanol effects could be the consequence of the alterations of these neurotransmitters/neuromodulatory systems.
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[Neuroscience] |
[Physiology] |
