Poster
# 16
Poster |
|
6th Internet World Congress for Biomedical Sciences |
Monica Acosta(1), Kiyohito Yoshida(2)
(1)(2)Hokkaido University - Sapporo. Japan
|
| ||
[Cell Biology & Cytology] |
[Genetics & Bioinformatics] |
Nomenclature and origin of the Om mutants. The Om mutants originate from the crossing of ca ( claret eye color, linked to chromosome 2) males and px (plexus wing venation, linked to chromosome 3) females. The suppressor mutants (Su) are symbolized like other Om mutants, for example Om(1J)Su34, where (1J) indicates the X-linked Om locus and 34 refers to a particular allele of the locus. The Om(1J)Su34 mutant stock employed in this investigation was established from the crossing of copper forked49 Beadex2 whiteg females and Om(1D)9 males (Hinton, unpublished data). The Om(1J)Su34 mutation suppress semidominantly the Om(1D)9 phenotype and an almost wild type eye is observed. The revertants flies of the Om(1J)Su34 gene, symbolized as Om(1J)Su34R, were obtained in the progeny of a single Om(1J)Su34 Om(1D)9 male, irradiated with 30 Gy of g-ray from a 60Co source (Yoshida, unpublished data). The Om(1J)Su34R Om(1D)9 flies were detected because of their Om(1D)9 phenotype. To stablish homocigotes Om(1J)Su34R mutants lines, the Om(1J)Su34R Om(1D)9/Om(1J)Su34R Om(1D)9 females were selected, however, in general, these flies presented low viability, did not lead viable progeny or did not lead eggs at all. Only the Om(1J)Su34R5 Om(1D)9 females allowed to maintain a homocigote line, but it was lost before the beginning of the analyses. The crossing of Om(1J)Su34R Om(1D)9 males to C1 f g/m v f g/Y females (compound X chromosome that has two linked X chromosomes that segregate together), did not exhibit enough fertility as to maintain an homozygous Om(1J)Su34R Om(1D)9 stock. The Om(1J)Su34R stock was then mantained by selecting the mutant revertant phenotype (that could be any of these genotypes: Om(1J)Su34R Om(1D)9/Om(1J)Su34 Om(1D)9, Om(1J)Su34R Om(1D)9/Om(1J)Su34R Om(1D)9 or Om(1J)Su34R Om(1D)9/Y), in each generation. The periodic examination of the Om(1J)Su34 Om(1D)9 stock, did not revealed any new spontaneous mutation or revertant of the Om(1J)Su phenotype.
Genetic mapping of the Om(1J)Su34R mutants. The Om(1J)Su34R mutations were genetically mapped by analyzing the recombination frequency in the yellow-Om(1J) interval, in the progeny of the crossing between Om(1J)Su34R Om(1D)9 males and homozygous cut yellow Om(1J)Su34 Om(1D)9 females, symbolized as ct y Om(1J) Om(1D). As control, it was analyzed the recombination frequency for this same interval for the Om(1J)Su34 Om(1D)9 stock crossed to the yellow Om(1D)9 stock (symbolized as y Om(1D)9). Culture conditions. All the stocks and the experimental crosses were cultured in a standard agar-yeast corn meal medium at 24C. For the experimental crosses, 3-4 day-old adult virgins of each sex were selected for mating.
Scanning electron microscopy (SEM). The eyes were prepared according to the technique of Kimmel (14).
General molecular procedures. General techniques protocols and solutions recipies were done according to Sambrook et al. (15).
in situ hybridization to the polytene chromosomes with DIG labeled probes. It was done according to the method of Engels et al. (16).
Southern blot. In the procedure of Southern blot it was employed the method of Pirrota (17) modified according to the Boheringer Mannheim catalog.
|
|
| ||
|
[Cell Biology & Cytology] |
[Genetics & Bioinformatics] |
