Jesus Gonzalez Garcia⁽¹⁾, Marcial García Rojo⁽²⁾, Francisco Martín Dávila⁽³⁾, Rafael López Pérez⁽⁴⁾, Margarita Delgado Portela⁽⁵⁾, Manuel Carbajo Vicente^{(6)
(1)(2)(3)(4)(5)(6)}Servicio de Anatomía Patológica. Complejo Hospitalario de Ciudad Real - Ciudad Real. Spain

	[Orthopedics & Traumatology]	[Pathology]

HISTOLOGIC AND IMMUNOPATHOLOGIC FEATURES

The specimen was fixed in 10% buffered formalin and sections were stained with H&E, PAS, Giemsa, Alcian blue and reticulin. Immunohistochemically study was performed with Cytokeratin cocktail, EMA, Vimentin, S100, bcl2, CD34 and MIC2 antibodies, using standard methods.

Goss and microscopic findings

The tumor sized 5x4x3 cm. and had an irregular contour with a polilobulated and something villous joint surface with fatty tissue on the opposite surface. It had a soft consistency and a homogeneous grey-yellowish cut surface (Fig. 2 and 3).

Microscopic sections revealed a dense proliferation of fusiform cells arranged in a lightly fasciculated pattern (Fig 4a and 4b) with other more loosely cellular areas (Fig. 5a and 5b) and others high vascularized (Fig. 6). It was relatively well-delimited from peritumoral tissues (Fig. 7a, b and c). The monomorphous tumoral cells were medium-size with elongated nucleus, low nuclear atypia, inconspicuous nucleolus and a mitotic rate of 4-7/10 hpf((Fig. 8a and b)). Frequently, clefts were observed (Fig. 9) partially lined by hyperplastic synovial tissue. Numerous mast cells were found (Fig 10). Microcalcifications (Fig. 11) and storiform microscopic structures were observed focally, sometimes with a mucous PAS-D and Alcian blue positive center (Fig. 12a and b, and 13).

Immunohistochemical study

Neoplastic cells showed a strong positivity to Vimentin (Fig. 14a), bcl2 (Fig. 14b), EMA (Fig. 14c) and PCNA (Fig. 14d). Lighter reactivity was observed with Factor XIIIa (Fig. 14e) and only focally with citokeratin cocktail (CK8, 18 and 19) (Fig. 14f) and MIC2. The cells were negative to S100 protein, CD34, CD68, and muscle-specific Actin.

Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [CLINICAL FEATURES] [HISTOLOGIC AND IMMUNOPATHOLOGIC FEATURES] [FIGURES] [FIGURES-2] [FIGURES-3] [FIGURES-4] [DISCUSSION] [REFERENCES] [Discussion Board]

---

	[Orthopedics & Traumatology]	[Pathology]

Jesus Gonzalez Garcia, Marcial García Rojo, Francisco Martín Dávila, Rafael López Pérez, Margarita Delgado Portela, Manuel Carbajo Vicente
Copyright © 1999-2000. All rights reserved.

Last update: 1/10/99