OLGA LARINA⁽¹⁾, Volodimir Manko^(2)
(1)DEPARTMENT OF HUMAN AND ANIMAL PHYSIOLOGY. I.FRANKO LVIV NATIONAL UNIVERSITY - LVIV . Ukraine
⁽²⁾Dept. Of Human and Animal Phisiology. I. Franko Lviv National University - Lviv. Ukraine

	[Endocrinology]	[Physiology]

METHODS

Experiments were performed on secretory cells of isolated salivary glands in Chironomus plumosus L. larva. The Na⁺-Ca²⁺ exchange current (I_Na(Ca)) was registered by means of voltage-clamp method in conditions of intracellular perfusion in response to the membrane hyperpolarization from –20 to –60 mV by rectangular pulses (3.5 sec, 0.1 Hz).

Solution for intracellular perfusion contained (in mmol/l): Tris-HCl, 130.14; NaCl, 16; glucose, 5.55; pH 7.0. Physiological extracellular solution contained (in mmol/l): NaCl, 136.9; KCl, 5.36; CaCl2, 1.76; MgCl2, 0.49; Tris-HCl, 0.20; glucose, 5.55; pH 7.2.

Alterations in extracellular Na⁺ concentration ([Na⁺]_e) were provided with equimolar substitution of NaCl with Tris-HCl. Changes in pH were produced by addition of HCl (in case of acidification to pH 5.0) and Tris-HCl in case of alkalization to pH 8, 8.5, 9 and 10.

Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [METHODS] [RESULTS] [IMAGES] [DISCUSSION] [CONCLUSIONS] [REFERENCES] [Discussion Board]

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	[Endocrinology]	[Physiology]

OLGA LARINA, Volodimir Manko
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Last update: 13/01/00