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6th Internet World Congress for Biomedical Sciences

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Role Of pH In Functioning Of Na+-Ca2+ Exchanger In Secretory Cell Plasma Membrane

OLGA LARINA(1), Volodimir Manko(2)
(1)DEPARTMENT OF HUMAN AND ANIMAL PHYSIOLOGY. I.FRANKO LVIV NATIONAL UNIVERSITY - LVIV . Ukraine
(2)Dept. Of Human and Animal Phisiology. I. Franko Lviv National University - Lviv. Ukraine

Discussion Board Contact address: OLGA LARINA
DEPARTMENT OF HUMAN AND ANIMAL PHYSIOLOGY I.FRANKO LVIV NATIONAL UNIVERSITY
LVIV
79066 Ukraine
larin@lreri.lviv.ua
[ABSTRACT] [INTRODUCTION] [METHODS] [RESULTS] [IMAGES] [DISCUSSION] [CONCLUSIONS] [REFERENCES] [Discussion Board]
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[Endocrinology]
Next: Effects of ethanol on aminopeptidase A in cortical synaptosomes.		 [Physiology]
Next: Effects of ethanol on brain aminopeptidase activities under basal and K+-stimulated conditions.

ABSTRACT

OBJECTIVE: The influence of alkalization and acidification of intra and(or) extracellular solution on INa(Ca) was studied.

METHODS:  INa(Ca)  in cells of salyvary gland  in  Chironomus plumosus larva  was  registered  by  means of voltage-clamp  method.

RESULTS: 

  1. Extracellular  solution  alkalization caused significant increase of INa(Ca) amplitude. Alkalization of  intracellular solution caused significant reduction of inward INa(Ca) amplitude and change of INa(Ca) direction from inward to outward[1]. Similar effect  was found when simultaneous alkalization of intra- and extracellular solutions took place.
  2. Change INa(Ca) amplitude was more significant at low [Na+]e and high [Ca2+]e.
  3. Extracellular acidification caused significant decrease of INa(Ca).

Reduction  pHe <  4 caused change of INa(Ca) direction from inward to outward. Change of  INa(Ca) evoked when reduction  of cytoplasmic pH and simultaneous reduction of intra- and  extracellular pH took place.

CONCLUSIONS:  Reaction  of binding Ca2+ with cation-transporting sites at cytoplasmic surface  of  the  exchanger in secretory cells is sensitive  to deprotonation. Protons promote displacement of Na+ by Ca2+ from exchanger molecule.

[1]. Manko V., Klevets M.,  Larina O., Second FEPS Congress Proceedings.

    -Physiol.Res.: Suppl.48(1):S 46 1999.

Keywords: receptor - cell biology - Calcium -
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[ABSTRACT] [INTRODUCTION] [METHODS] [RESULTS] [IMAGES] [DISCUSSION] [CONCLUSIONS] [REFERENCES] [Discussion Board]
Main Page Previous: Vestibular Evoked Potentials with Caloric Stimulation INTRODUCTION
[Endocrinology]
Next: Effects of ethanol on aminopeptidase A in cortical synaptosomes.		 [Physiology]
Next: Effects of ethanol on brain aminopeptidase activities under basal and K+-stimulated conditions.
OLGA LARINA, Volodimir Manko
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