Presentation
# 2
Presentation |
|
6th Internet World Congress for Biomedical Sciences |
S. Dhakshinamoorthy(1), D. Bloom(2), Anil Jaiswal(3)
(1)(2)Department of Pharmacology. Baylor College of Medicine. - Houston. United States
(3)Baylor College of Medicine - Houston. United States
|
 |
|
 |
 |
|
Fig. 1. Initial and accumulatory effects of chemically induced superoxide and electrophiles.
|
 |
|
Fig. 2. Antioxidant and xenobiotic inducible genes.
|
 |
|
Fig. 3. Alignment of antioxidant response elements (AREs) from seven genes. The TRE and TRE-like elements, their orientations and ´GC´ box are indicated. ARE core sequence was determined by alignment and mutational studies.
|
 |
|
Fig. 4. Antioxidant response element (ARE) binding proteins. A. Human NQO1 gene ARE-binding proteins. B. Important functional domains of NF-E2 related factors (Nrf1 and Nrf2). CNC, Cap and collar domain.
|
 |
|
Fig. 5. Effect of overexpression of nuclear proteins Nrf1 and Nrf2 on hARE-mediated CAT gene expression. The Hep-G2 cells were co-transfected with 10 mg of reporter plasmid hARE-tk-CAT (left panel) and various concentrations (mg) of expression LNCX-Nrf1R and LNCX-Nrf2R (containing Nrf1 and Nrf2 cDNA in reverse orientation) and LNCX-Nrf1C and LNCX-Nrf2C (containing Nrf1 and Nrf2 cDNA in correct orientation) in separate experiments as shown. Five mg of RSV-b-galactosidase plasmid was used in each case as control of transfection efficiency. Forty eight hours after the transfection, the cells were analyzed for b-galactosidase and CAT activities.
|
|
|
|
|
|
|
