Poster
# 79
Poster |
|
6th Internet World Congress for Biomedical Sciences |
John Cassella(1), Joseph Kelly(2)
(1)(2)School of Radiography & Podiatry. University of Central England - Birmingham. United Kingdom
 |
Contact address: |
John Cassella School of Radiography & Podiatry University of Central England Frachise Street, Perry Barr Birmingham B42 2SU United Kingdom John.Cassella@UCE.ac.uk |
|
 |
|
|
|
[Physiology] |
[Neurology] |
[Nutrition & Diabetics] |
Many aspects of the normal response of the foot to injury are reduced or absent in diabetes. The integrity of endothelial smooth muscle combined with neuropathy has been proposed as a mechanism involved in the pathogenisis of foot ulceration. The present study investigated the involvement of peripheral sensory neuropathy in endothelial dependent and endothelial independent vasodilation in three groups of age and sex matched subjects. n=18; six NIDDM subjects with diabetic neuropathy, six NIDDM subjects without neuropathy and six non diabetic controls. Dorsal foot skin blood flow was measured by laser Doppler flowmetry in response to iontophoresis of 1% Acetylcholine (ACh -Dependent response) and 1% Sodium nitroprusside (SNP - Independent response). Previous studies have employed low currents and long durations of iontophoresis. In this present study a constant high current (40 milliamps) and short (9x60 seconds) iontophoresis duration protocol was used. This provided a cumulative effect over time. The transcutaneous oxygen tension (TCPO2) between groups was also determined. Mean baseline arbitrary unit (AU) values were; 3.7+/-1.6 in the diabetic group, 4.3+/- 2.6 in the diabetic control and 4.2+/- 2.5 in the healthy controls respectively. After iontophoretic administration, the peak values recorded were; diabetic group 26.5+/- 10.7; diabetic control 16.1+/- 8.4 and healthy control 27.0+/- 8.6. A significant difference between main effect for time within each group was found. (p<0.001 2-way ANOVA). Analysis of SNP mean values at baseline were diabetic experiment 6.0+/-2.4, diabetic control 7.7 +/-4.1 and healthy control 9. 1 +/- 7.0 AU´s respectively. Peak recorded values were; diabetic group 38.2 +/-18.4, diabetic control 44.3 +/-24.1 and healthy control 49.8+/- 18.7AU´s. Significance was reached for time (p<0.001) but not for group by time interaction. Analysis of PO2 values reached significance in correlation with ACh (r = 0.60, p<0.01) but not SNP. This study supports the hypotheses of current induced hyperaemia or denervation hypersensitivity previously reported. In conclusion further investigation with high currents in neuropathic subjects other than those with diabetes is needed and may be useful in evaluating the exact contribution of any current effect or non-specific effects.
|
|
|
|
|
|
|
[Physiology] |
[Neurology] |
[Nutrition & Diabetics] |
