María Jesús García-López⁽¹⁾, María Jesús Ramírez-Expósito⁽²⁾, José Manuel Martínez-Martos⁽³⁾, María Dolores Mayas-Torres⁽⁴⁾, Isabel Prieto-Gómez⁽⁵⁾, Garbiñe Arechaga-Maza⁽⁶⁾, Manuel Ramírez-Sánchez^{(7)
(1)(3)(4)(5)(6)(7)}Unit of Physiology. University of Jaén - Jaén. Spain
⁽²⁾Unit of Physiology. University of Jaen - Jaén. Spain

	[Biochemistry]	[Endocrinology]	[Neuroscience]	[Physiology]

RESULTS

Figure 1 shows the mean±SEM of serum and soluble and membrane bound pGluAP activity in adrenal glands, hypophisis, frontal cortex and hypothalamus in all the experimental groups considered.

In serum orchiectomy caused a increase of activity (p<0.05). Testosterone in E1 and E2 did not change the activity, however, pGluAP activity in E3 group decreased significantly (p<0.01) in relation with orchiectomized mice.

In adrenal glands soluble pGluAP activity did not change with orchiectomy. This activity changed after the injection of dose 1. E1 group was significantly lower than control (p<0.05). No variations were observed between control groups and rest of all the groups in membrane bound pGluAP.

In hypophisis, soluble pGluAP activity increased significantly (p<0.05) after orchiectomy. This activity in E1 group was higher than in controls (p<0.01) and than in ORX (p<0.05). A decrease dose-dependent was observed in pGluAP activity after the injection of doses 2 and 3. membrane bound activity did not show differences after orchiectomy and after injection of testosterones.

In frontal cortex, orchiectomy did not induce differences in soluble pGluAP activity. The injection of testosterone neither produced changes in soluble pGluAP activity. In membrane bound pGluAP activity, no changes were observed after orchiectomy. E3 groups is the oly group where membrane bound pGluAP activity was modified. Lower levels of activity in relation with control (p<0.01) and ORX (p<0.05) was observed in E3 group.

In Hypothalamus, in E2 groups, soluble pGluAP activity was higer than in controls and in ORX group (p<0.05). No changes were observed betwee the other groups. Membrane bound pGluAP activity was similar in all the experimental groups. Orchiectomy and injection of testosterone did not induce differences between the groups.

Discussion Board

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[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION] [CONCLUSIONS] [BIBLIOGRAPHY] [Discussion Board]

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	[Biochemistry]	[Endocrinology]	[Neuroscience]	[Physiology]

María Jesús García-López, María Jesús Ramírez-Expósito, José Manuel Martínez-Martos, María Dolores Mayas-Torres, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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Last update: 15/01/00