Poster
# 47
Poster |
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6th Internet World Congress for Biomedical Sciences |
Sándor Losonczy(1), Csaba Szabó(2), Zsuzsnna Kiss(3), László Bárdos(4)
(1)(2)(3)(4)Animal Physiology and Health - Gödöllő. Hungary
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[Biochemistry] |
[Immunology] |
The main immunoglobulin fraction of fowls is called IgY, in order to distinguish it from the mammalian IgG. There are some crucial points of difference between IgG and IgY. Avian IgY is more resistant to heat, low pH and/or ion strength of environment than IgG. IgY has no reactivity to mammalian auto-antibodies, Fc-receptors and red blood cells fig.1. In assays, these cross-reactions can be eliminated using avian-originated antibodies (ABs). Serum IgY is identical to yolk AB, as it has been proven in comparative studies. The isolation and purification for further different uses of AB has great potentials. The extraction and purification of immunoglobulin fraction (IgY) from egg yolk of domestic hens and Japanese quail hens is described earlier (4,5,8).
According to the literature (1,4) and our experience (5), advantages of the IgY extracted from egg yolk are the following: 1. birds (chicken, quail etc.) produce ABs against highly conservative mammalian protein, too; 2. the amount of antigen needed for immune response is very low; 3. collecting and storing eggs are non-invasive and not expensive in contrast to bleeding animals and deep frozen storage of sera; 4. AB isolation and purification of yolks by combined methods are quick and inexpensive, 5. the avian AB is acid- and heat resistant, for these reasons it might be used for oral immunotherapy as well.
With regard to animal welfare (i.e. blood sampling), ABs, purified from egg yolks of immunized chickens can play an increasing role as an alternative to classic (mammalian) ABs. They can be used in immunodiagnostics (1,6) and therapy as well (9,10).
In the field of immunology the Japanese quail as a model animal could have an increasing importance. But it should be mentioned that specific anti quail antibodies (ABs) are not aviable on the market, although the production of AB in Japanese quail has special benefits. The quail’s advantages are smaller body weigh, less food intake and smaller room in the batteries. Sexual maturity of the female quail is 35-40 days in contrast with that of the domestic hen, which starts laying at the age of 130-150 days.
The development of a sensitive ELISA for the measurement of quail IgY (QIgY) was the main purpose of our study.
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[Biochemistry] |
[Immunology] |
