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6th Internet World Congress for Biomedical Sciences

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Quantitative changes in neuronal population during aging and contralateral lesions.

María Jesús Ramírez-Expósito(1), José Manuel Martínez-Martos(2)
(1)Unit of Physiology. University of Jaen - Jaén. Spain
(2)Unit of Physiology. University of Jaén - Jaén. Spain

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]
MATERIAL & METHODS Previous: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture. Previous: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture. Previous: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture. IMAGES
[Cell Biology & Cytology]
Next: Quantitative changes in glial population during aging and contralateral lesions.
[Neuroscience]
Next: Quantitative changes in glial population during aging and contralateral lesions.
[Physiology]
Next: Quantitative changes in glial population during aging and contralateral lesions.

RESULTS

shows numerical cell density (mean±SEM) obtained in the different cortical layers of the Fr zone. No significant differences were observed in the different cortical layers in the number of TN. The study of the different neuronal types considered showed a significant (p<0.001) decrease in the number of NN in layer II-IV, V and VI of lesioned animals although no differences were observed for layer I. By contrast, in all layers, lesioned animals showed a significant increase (p<0.001) of SDN. No changes were observed in the number of LDN with the lesion.

The study of the influence of contralateral lesions in the neuronal soma, nucleus and nucleolus area are showed in figure 3. Neuronal soma area showed a significant (p<0.001) increase in the TN of lesioned animals. Considering the different neuronal types, the soma area of NN were higher (p<0.001) in lesioned animals than in controls. No changes were observed in the soma area of LDN and SDN with the lesion. In all the studied animals, the soma area of LDN and SDN is significantly lower (p<0.001) than the soma area of NN. No differences were observed in the soma form index of TN, NN, LDN and SDN between lesioned and control animals (figure 4). Taken together, SDN presented a higher soma form index (p<0.001) than LDN, and these higher than NN (p<0.001).

The study of the nucleus area (figure 3) showed a lesion-dependent increase (p<0.001) in NN, whereas no significant differences were observed in nucleus area of LDN and SDN between lesioned and control rats. However in all the studied animals, the nucleus area of LDN and SDN were significantly (p<0.001) lower than nucleus area of NN. The nucleus area of TN did not change with the contralateral lesions. No lesion-dependent differences were observed in the nucleus form index in NN, LDN, SDN and NT. Taken together, SDN presented a higher (p<0.001) nucleus form index than LDN and these, higher (p<0.001) than NN.

The study of the nucleolus area showed a significant increase (p<0.001) in NN of lesioned animals. No changes were detected in LDN and SDN with the lesion. The nucleolus area of LDN and SDN was significantly lower than nucleolus area of NN.


Discussion Board
Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [IMAGES] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]

MATERIAL & METHODS Previous: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture. Previous: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture. Previous: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture. IMAGES
[Cell Biology & Cytology]
Next: Quantitative changes in glial population during aging and contralateral lesions.
[Neuroscience]
Next: Quantitative changes in glial population during aging and contralateral lesions.
[Physiology]
Next: Quantitative changes in glial population during aging and contralateral lesions.
María Jesús Ramírez-Expósito, José Manuel Martínez-Martos
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Last update: 10/01/00