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6th Internet World Congress for Biomedical Sciences

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Utility of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to measure mitochondrial activity in K+- and ATP- stimulated rodent cortex synaptosomes.

José Manuel Martínez-Martos(1), María Jesús Ramírez-Expósito(2), María Dolores Mayas-Torres(3), Isabel Prieto-Gómez(4), Manuel Ramírez-Sánchez(5)
(1)(3)(4)(5)Unit of Physiology. University of Jaén - Jaén. Spain
(2)Unit of Physiology. University of Jaen - Jaén. Spain

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]
RESULTS Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. DISCUSSION
[Neuroscience]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.
[Physiology]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.

FIGURES

Figure 1
Figure 1. Influence of increased protein content of rat (A) and mouse (B) cortex synaptosomes on MTT reduction after one or two hours of incubation at 37°C. Results are expressed as mean±SEM of eight replicates per point.

 

Figure 2
Figure 2. MTT cleavage by rat (A) and mouse (B) cortex synaptosomes under basal (control, C) and K+-stimulated conditions after one (continuous line) or two hours (discontinuous line) of incubation at 37°C; ( , represents a protein content of the synaptosomal fraction of 1 mg/ml; , represents a protein content of the synaptosomal fraction of 2 mg/ml). Results are shown as the mean±SEM of eight replicates per point.

 

Figure 3
Figure 3. MTT cleavage by rat (A) and mouse (B) cortex synaptosomes under basal (control, C) and ATP-stimulated conditions after one (continuous line) or two hours (discontinuous line) of incubation at 37°C; ( , represents a protein content of the synaptosomal fraction of 1 mg/ml; , represents a protein content of the synaptosomal fraction of 2 mg/ml). Results are shown as the mean±SEM of eight replicates per point.

 


Discussion Board
Discussion Board

Any Comment to this presentation?

[ABSTRACT] [INTRODUCTION] [MATERIAL & METHODS] [RESULTS] [FIGURES] [DISCUSSION] [BIBLIOGRAPHY] [Discussion Board]

RESULTS Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. Previous: In vitro study on the effect of ethanol on basal and stimulated pyroglutamyl aminopeptidase activity in mouse brain. DISCUSSION
[Neuroscience]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.
[Physiology]
Next: Differential effects of exogenous oleic and linoleic fatty acids and cholesterol on aminopeptidase activities in rat astrocytes in primary culture.
José Manuel Martínez-Martos, María Jesús Ramírez-Expósito, María Dolores Mayas-Torres, Isabel Prieto-Gómez, Manuel Ramírez-Sánchez
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Last update: 16/12/99