Poster
# 12
Poster |
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6th Internet World Congress for Biomedical Sciences |
Montserrat Mitjans(1), Liseth Garcia(2), Evangelina Marrero(3), Pilar Vinardell(4)
(1)(4)Departament de Fisiologia-Div IV. Facultat de Farmacia - Barcelona. Spain
(2)(3)Centro Nacional de Sanidad Agropecuaria - San José de las Lajas. Cuba
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[Gastroenterology & Hepatology] |
[Pathology] |
The equipment, handling and sacrifice of animals was performed in accordance with the European Council Legislation 87/609/EC for the protection of experimental animals. Male Wistar rats (Harlam Interfauna Iberica, S.A., Barcelona, Spain) with an initial weight of about 210 g were housed in cages in an animal room maintained at 22 ± 2ºC with a relative humidity of 60 ± 15% and 12-12 hours light-dark cycle. The animals were fed ad libitum with laboratory chow and allowed free access to tap water. To avoid the effect of diurnal changes, administration and other operations were performed at 14:00 h. Eight treated animals received a daily oral dose of 2g/Kg of Ligmed-A for 4 days, because it is the recommended dose and periodicity of the administration (5). The compound was suspended in distilled water and a volume of 10 ml/Kg was intragastrically administrated. Four control animals received 10 ml/Kg of distilled water. At the end of the treatment period the animals were anesthetized and the whole small intestine was quickly removed and divided into duodenal, jejunal and ileal portions, washed with ice-cold saline, and then fixed in 10% formalin at room temperature for 48 h. Tissue pieces were then rinsed in tap water for 1 h and dehydrated in a graded ethanol series, embedded in paraffin, and cut into 5-micrometer sections along the villus-crypt axis. Paraffin was removed from the slices by placing them in xylene followed by a graded ethanol series, and finally they were stained with hematoxylin/eosin for microscopic examination. Eight to ten samples from each animal and section were observed with a Leica microscope equipped with a video camera. The images were load and then treated with a image computer program (IMAT) developped by the Serveis Cientifico-Tècnics of Barcelona University. In each section wall thickness, villus length and width, crypt depth (invagination between adjacent villi)(Figure 1), villus linear density and ratio C:V (number of crypt for each villi) (6-8)were determined.
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[Gastroenterology & Hepatology] |
[Pathology] |
