María Jesús García-López⁽¹⁾, María Jesús Ramírez-Expósito⁽²⁾, José Manuel Martínez-Martos⁽³⁾, María Dolores Mayas-Torres⁽⁴⁾, Isabel Prieto-Gómez⁽⁵⁾, Garbiñe Arechaga-Maza⁽⁶⁾, Manuel Ramírez-Sánchez^{(7)
(1)(3)(4)(5)(6)(7)}Unit of Physiology. University of Jaén - Jaén. Spain
⁽²⁾Unit of Physiology. University of Jaen - Jaén. Spain

Contact address:

María Jesús García-López Unit of Physiology University of Jaén Faculty of Experimental and Health Sciences Jaén E-23071 Spain jmmartos@ujaen.es

					[Reproduction Sciences]
	[Biochemistry]	[Endocrinology]	[Neuroscience]	[Physiology]

ABSTRACT

The effects of orchiectomy and testosterone on soluble (S) and membrane bound (MB) Alanine (Ala-), Arginine (Arg-) and Cysteine (Cys-) aminopeptidase (AP) activities were studied in serum and frontal cortex (FC), Hypothalamus (Ht) , Hypophysis (Hp) and adrenal glands (AG) of mice. Forty male mice were distributed in five groups: Control (C), orchiectomized (ORX) and orchiectomized and injected with different doses of testosterone (E1, E2, E3). In serum, AlaAP and CysAP did not change, but ArgAP activity decreased after orchiectomy. The injection of testosterone induced an increase in ArgAP activity. In FC, orchiectomy produced a significant decrease in soluble CysAP only. Testosterone induced a significant increase in soluble AlaAP. Higher soluble ArgAP and CysAP activities were observed after injection of doses E2 and E3. In Ht, the soluble aminopeptidases analysed, did not change after orchiectomy, but the injection of dose E2 of testosterone produced significant increases in soluble AlaAP, ArgAP and CysAP. However, MB AlaAP, ArgAP and CysAP activities increase in ORX group. Significant changes were observed in these AP activities after injection of testosterone. Soluble AlaAP, ArgAP, and CysAP were increased in Hp in all the groups analysed. In AG, no changes were observed in S and MB AlaAP, ArgAP and CysAP activities after orchiectomy, but differences were observed between E1, E2 and E3 groups and control animals. These results confirm the previous hypothesis of an hormonal influence on AP activity.

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Keywords: Alanyl aminopeptidase - Arginyl aminopeptidase - Cystinil aminopeptidase - Orchiectomy - Testosterone -

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					[Reproduction Sciences]
	[Biochemistry]	[Endocrinology]	[Neuroscience]	[Physiology]

María Jesús García-López, María Jesús Ramírez-Expósito, José Manuel Martínez-Martos, María Dolores Mayas-Torres, Isabel Prieto-Gómez, Garbiñe Arechaga-Maza, Manuel Ramírez-Sánchez
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Last update: 15/01/00