Poster
# 69
Poster |
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6th Internet World Congress for Biomedical Sciences |
Stephan Lolov(1), Dinka Petrova(2), Stanimir Kyurkchiev(3), George Edrev(4)
(1)Institute of Biology and Immunology of Reproduction. Bulgarian Academy of Sciences - Sofia. Bulgaria
(2)(3)Institute of Biology and Immunology of Reproduction; Bulg. Acad. Sci. - Sofia. Bulgaria
(4)Dept. Otorhinolaryngology. Transport Medical Institute - Sofia. Bulgaria
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Contact address: |
Stephan Lolov Institute of Biology and Immunology of Reproduction Bulgarian Academy of Sciences 73 Tzarigradsko shousse Sofia 1113 Bulgaria lolov@mont.nbu.acad.bg |
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[Immunology] |
[Otorhinolaryngology] |
Cholesteatoma is a destructive lesion of the human middle ear with unrevealed etiopathogenesis. Immunohistochemical assays with specific monoclonal antibodies have been used to register expression and distribution of several well-known antigens. In this study, the authors have looked for abnormal or unknown cholesteatoma’s constituents generating monoclonal antibodies.
BALB/c mice were immunized with subcutaneous implantation of the cholesteatomas’ sac and matrix. Hybridomas have been generated following procedures known in the art. The screening for clones with specific antibodies production was done using ELISA against salt-water tissue extract and IF with paraffin embedded tissue sections.
The monoclonal antibody named 1F8 showed positive reaction in ELISA and in IF when tested against both cholesteatoma and ear canal skin. At the same time when supernatants from different cell and tissue cultures have been used as an antigen in ELISA, IF8 reacted only with cholesteatomas’ supernatant. In Western blot it recognizes three small bands and a prominent one, with MW of approximately 70 kD. Using immunohistochemistry the antigen was localized in the subepithelial connective tissue fibers of the skin and the cholesteatoma. The antigen of IF8 was detected in all tested cholesteatomas. Studies are now in progress to characterize the antigen in more details.
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[Immunology] |
[Otorhinolaryngology] |
