Poster
# 25
Poster |
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6th Internet World Congress for Biomedical Sciences |
María Jesús Ramírez-Expósito(1), José Manuel Martínez-Martos(2)
(1)Unit of Physiology. University of Jaen - Jaén. Spain
(2)Unit of Physiology. University of Jaén - Jaén. Spain
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Contact address: |
María Jesús Ramírez-Expósito Unit of Physiology University of Jaen Faculty of Experimental and Health Sciences Jaén E-23071 Spain mramirez@ujaen.es |
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[Cell Biology & Cytology] |
[Neuroscience] |
[Physiology] |
Introduction. Glial cells are characterized by a high capacity of reaction during lesion and also in aging. This normal reactivity usually is accompained by proliferation. Astrocytes and also microglia cells look the most affected cells during these proccess. The aim of this work is to analyzed the possible quantitative changes of glial population during aging and contralateral lesions, and the distribution of these variations in the different cortical layers. Material and Methods. Three groups of male Wistar rats (Control, aging animals and aging and estereotaxic lesioned animals) were used. After the sacrifice and perfussion of the animals, the brains were obtained and semithin sections were obtained and stained with toluidine blue. On these semithin sections, counts were made using micrometer-ocular techniques. Two different groups of glial cells were considered: Astrocytes and microglia-oligodendrocytes. Results. Aging produced an increase in the number of glial cells in cortical layer V. However, the lesion produced a generalized increase in all the cortical layers (except layer VI). In old animals, the astrocytes were the glial cell type most affected, but, in lesioned animals, the number of glial cells were increased by the microglia-oligodendrocytes. Conclusion. Although ageing and lesions produce an increase in the total glial population, in each proccess the type of glial cell affected is different. The localization of these changes is different too: Aging is a more focused proccess (layer V) but lesions affected to all cortical layers.
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[Cell Biology & Cytology] |
[Neuroscience] |
[Physiology] |
