Poster
# 22
Poster |
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6th Internet World Congress for Biomedical Sciences |
José Manuel Martínez-Martos(1), María Jesús Ramírez-Expósito(2), María Dolores Mayas-Torres(3), Isabel Prieto-Gómez(4), Manuel Ramírez-Sánchez(5)
(1)(3)(4)(5)Unit of Physiology. University of Jaén - Jaén. Spain
(2)Unit of Physiology. University of Jaen - Jaén. Spain
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Contact address: |
José Manuel Martínez-Martos Unit of Physiology University of Jaén Faculty of Experimental and Health Sciences Jaén E-23071 Spain jmmartos@ujaen.es |
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[Neuroscience] |
[Physiology] |
Although tetrazolium salt 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay has been widely used to measuring cell proliferation and cytotoxicity, our aim was to check the utility of the MTT assay to measure mitochondrial activity as an index of the energetic status of resting and stimulated rodent cortical synaptosomes. Synaptosomes were obtained from the frontal cortex of adult rats and mice. MTT assay was performed under basal (resting) conditions and under stimulation with KCl (25, 50 and 100 mM) and ATP (1, 2, 4, 10 and 20 µM). MTT reduction was linear with the synaptosomal protein content. No differences were found in the levels of MTT reduction between rats and mice. K+-stimulation increases MTT reduction in both rat and mice synaptosomes, reaching the higher values under 50 mM K+ stimulus. Stimulation with ATP increased MTT reduction of both rat and mouse synaptosomes at low doses (1-4 µM), whereas high doses (10-20 µM) decreased MTT reduction under control levels in both cases. MTT assay could be a suitable, quick and easy method to measure mitochondrial activity as an index of the energetic behaviour of synaptosomes under resting and/or stimulating conditions in different experimental protocols.
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[Neuroscience] |
[Physiology] |
