Comunicación
      Nº 046

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Cuantificación de Ácidos Nucléicos Desarrollados en Gel de Agarosa. Diseño y Validación de un Método Simple de Análisis Digital de Imagen.


Comentarios
[Título] [Introducción] [Material y Métodos] [Resultados] [Discusión] [Iconografía]

Bibliografía

1 - Herrington CS, Mc Gee JO. Diagnostic molecular pathology. A practical approach. Volumen I y II. Nueva York, NY: Oxford University Press, 1992.

2 - Newton CR, Graham A.Quanttative PCR. En: Billington D, editor. Introduction to biotechniques. PCR. Oxford, Reino Unido: BIOS Scientific Publischers Limited, 1997.

3 - Andrews AT. Electrophoresis, theory, techniques and biochemical and clinical applications. Nueva York: Oxford University Press, 1986.

4 - Kysela BP, Michael BD, Arrand JE. Relative contributions of levels of initial DNA damage and repair of double-strand bresks to the ionizing radiation sensitive phenotype of the chinese hamster cell mutant; XR-V15B. Part I, X rays. Int J Radiat Biol 1993;63:609-616.

5 - Iliakis GE, Metzger L, Denko N, Stamato, TD. Detection of DNA double-strand breaks in synchronous cultures of CHO cells by means of asymmetric field inversion gel electrophoresis. Int J Radiat Biol 1991;59:321-341.

6 - Andrews AT. Electrophoresis of nucleic acids. En: Brown TA, editor . Essential molecular biology. Apractical approach. Volumen I. Nueva York: Oxford University Press, 1991.

7 - Harriman WD, Wabl M. A video technique for quantification of DNA in gels stained with ethidium bromide. Anal Biochem 1995;228:336-342.

8 - Sutherland JC, Lin B, Monteleone DC, Mugavero J, Sutherland BM, Truck A. Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels: application to ethidium bromide-stained DNA. Ana Biochem 1987;163:446-457

9 - Chen CZ, Sutherland JC. Gel electrophoresis method for quantitation of gamma ray induced single- and double-strand breaks in DNA irradiated in vitro. Electrophoresis 1989;10,318-326.

10 - Uchida S, Takemoto F, Ogada E, Kurokawa K. Detection of endothelin-1 mRNA by RT-PCR in isolate rat renal tubules. Biochem Biophys Res Commun 1992;188(1):108-113.

11 - Nuñez DJ, Taylor EA, Oh VM, Schofield JP, Brown MJ. Endothelin-1 mRNA expression in the rat kidney. Biochem J 1991;1275(3):817-819.

12 - Gonzales RC, Woods RE. Digital image processing. Reading, MA: Addison-Wesley Publishing Company, 1992.

13 - Serra J. Image analysis and mathematical morphology. Londres, Reino Unido: Academic Press, 1982.

14 - Serra J. Image analysis and mathematical morphology: theorical advances. Londres, Reino Unido: Academic Press, 1988.

15 - Masseroli M, Messori A, Bendotti C, Ponti M, Forloni G. Automatic quantitative evaluation of autoradiographic band films by computerized image analysis. Life Sciencies 1993;53:331-336.

 

[Título] [Introducción] [Material y Métodos] [Resultados] [Discusión] [Iconografía]


Francisco Arrebola, MS.
Copyright © 1996, Departamento de Anatomía Patológica, Facultad de Medicina y Hospital Universitario, Universidad de Granada, 18012 Granada, España. Reservados todos los derechos.